Article (Scientific journals)
The contribution of uncoupling protein and ATP synthase to state 3 respiration in Acanthamoeba castellanii mitochondria.
Jarmuszkiewicz, Wieslawa ; Czarna, M.; Sluse-Goffart, Claudine et al.
2004In Acta Biochimica Polonica, 51, p. 533-538
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Keywords :
Mitochondria; uncoupling protein; energy-dissipating; acanthamoeba castellanii
Abstract :
[en] Mitochondria of the amoeba Acanthamoeba castellanii possess a free fatty acid-activated uncoupling protein (AcUCP) that mediates proton re-uptake driven by the mitochondrial proton electrochemical gradient. We show that AcUCP activity diverts energy from ATP synthesis during state 3 mitochondrial respiration in a fatty acid-dependent way. The efficiency of AcUCP in mitochondrial uncoupling increases when the state 3 respiratory rate decreases as the AcUCP contribution is constant at a given linoleic acid concentration while the ATP synthase contribution decreases with respiratory rate. Respiration sustained by this energy-dissipating process remains constant at a given linoleic acid concentration until more than 60% inhibition of state 3 respiration by n-butyl malonate is achieved. The present study supports the validity of the ADP/O method to determine the actual contributions of AcUCP (activated with various linoleic acid concentrations) and ATP synthase in state 3 respiration of A.castellanii mitochondria fully depleted of free fatty acid-activated and describes how the two contributions vary when the rate of succinate dehydrogenase is decreased by succinate uptake limitation
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Jarmuszkiewicz, Wieslawa ;  Université de Liège - ULiège
Czarna, M.
Sluse-Goffart, Claudine;  Université de Liège - ULiège
Sluse, Francis ;  Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
Language :
English
Title :
The contribution of uncoupling protein and ATP synthase to state 3 respiration in Acanthamoeba castellanii mitochondria.
Publication date :
2004
Journal title :
Acta Biochimica Polonica
ISSN :
0001-527X
eISSN :
1734-154X
Publisher :
Panstwowe Wydawnictwo Naukowe, Warsaw, Poland
Volume :
51
Pages :
533-538
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 December 2009

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