Reference : Identification and characterization of a protozoan uncoupling protein in Acanthamoeba ca...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/33005
Identification and characterization of a protozoan uncoupling protein in Acanthamoeba castellanii.
English
Jarmuszkiwicz, W. [ > > ]
Milani, G. [ > > ]
Fortes, F. [ > > ]
Schreiber, A. Z. [ > > ]
Sluse, Francis mailto [Université de Liège - ULg > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
Vercesi, A. [ > > ]
2000
FEBS Letters
Elsevier Science
467
145-149
Yes (verified by ORBi)
0014-5793
Amsterdam
The Netherlands
[en] yeast mitochonrion ; uncoupling protein ; candida parapsilosis
[en] An uncoupling protein (UCP) was identified in mitochondria from Candida parapsilosis (CpUCP), a non-fermentative parasitic yeast. CpUCP was immunodetected using polyclonal antibodies raised against plant UCP. Activity of CpUCP, investigated in mitochondria depleted of free fatty acids, was stimulated by linoleic acid (LA) and inhibited by GTP. Activity of CpUCP enhanced state 4 respiration by decreasing DeltaPsi and lowered the ADP/O ratio. Thus, it was able to divert energy from oxidative phosphorylation. The voltage dependence of electron flux indicated that LA had a pure protonophoretic effect. The discovery of CpUCP proves that UCP-like proteins occur in the four eukaryotic kingdoms: animals, plants, fungi and protists.
http://hdl.handle.net/2268/33005

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