Reference : Cyanide-resistant, ATP-synthesis-sustained, and uncoupling-protein-sustained respirat...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Cyanide-resistant, ATP-synthesis-sustained, and uncoupling-protein-sustained respiration during postharvest ripening of tomato fruit
Almeida, A. [ > > ]
Jarmuszkiewicz, W. [ > > ]
Khomsi, H. [ > > ]
Arruda, P. [ > > ]
Vercesi, A. [ > > ]
Sluse, Francis mailto [Université de Liège - ULg > Département des sciences de la vie > Bioénergétique et physiologie cellulaire >]
Plant Physiology
American Society of Plant Biologists
Yes (verified by ORBi)
[en] Cyanide-resistant ; ATP-synthesis ; Uncoupling protein ; Respiration ; Postharvest ripening ; tomato fruit
[en] Tomato (Lycopersicon esculentum) mitochondria contain both alternative oxidase (AOX) and uncoupling protein as energy-dissipating systems that can decrease the efficiency of oxidative phosphorylation. We followed the cyanide (CN)-resistant, ATP-synthesis-sustained, and uncoupling-protein-sustained respiration of isolated mitochondria, as well as the immunologically detectable levels of uncoupling protein and AOX, during tomato fruit ripening from the mature green stage to the red stage. The AOX protein level and CN-resistant respiration of isolated mitochondria decreased with ripening from the green to the red stage. The ATP-synthesis-sustained respiration followed the same behavior. In contrast, the level of uncoupling protein and the total uncoupling-protein-sustained respiration of isolated mitochondria decreased from only the yellow stage on. We observed an acute inhibition of the CN-resistant respiration by linoleic acid in the micromolar range. These results suggest that the two energy-dissipating systems could have different roles during the ripening process.

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