Article (Scientific journals)
Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.
Letellier, Carine; Boxus, Mathieu; Rosar, Laurent et al.
2008In Vaccine, 26 (37), p. 4840-8
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Keywords :
Animals; Bronchoalveolar Lavage Fluid/virology; CD8-Positive T-Lymphocytes/immunology; Cattle; Cattle Diseases/prevention & control; Cell Proliferation; Immunization, Secondary/methods; Interferon-gamma/biosynthesis; Lung/pathology/virology; Nucleocapsid Proteins/immunology; Plasmids; Pneumonia/prevention & control/veterinary; Respiratory Syncytial Virus Infections/prevention & control/veterinary; Respiratory Syncytial Virus Vaccines/immunology; Respiratory Syncytial Virus, Bovine/immunology; Vaccines, DNA/immunology; Vaccines, Subunit/immunology
Abstract :
[en] Respiratory syncytial virus (RSV) is a major cause of respiratory disease in both cattle and young children. Despite the development of vaccines against bovine (B)RSV, incomplete protection and exacerbation of subsequent RSV disease have occurred. In order to circumvent these problems, calves were vaccinated with the nucleocapsid protein, known to be a major target of CD8(+) T cells in cattle. This was performed according to a DNA prime-protein boost strategy. The results showed that DNA vaccination primed a specific T-cell-mediated response, as indicated by both a lymphoproliferative response and IFN-gamma production. These responses were enhanced after protein boost. After challenge, mock-vaccinated calves displayed gross pneumonic lesions and viral replication in the lungs. In contrast, calves vaccinated by successive administrations of plasmid DNA and protein exhibited protection against the development of pneumonic lesions and the viral replication in the BAL fluids and the lungs. The protection correlated to the cell-mediated immunity and not to the antibody response.
Disciplines :
Immunology & infectious disease
Microbiology
Veterinary medicine & animal health
Author, co-author :
Letellier, Carine
Boxus, Mathieu ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Rosar, Laurent
Toussaint, Jean*-Francois
Walravens, Karl
Roels, Stefan
Meyer, G.
Letesson, Jean*-Jacques
Kerkhofs, Pierre
Language :
English
Title :
Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.
Publication date :
2008
Journal title :
Vaccine
ISSN :
0264-410X
eISSN :
1873-2518
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
26
Issue :
37
Pages :
4840-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 December 2009

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