Article (Scientific journals)
Quantification of Randomly-methylated-b-cyclodextrin effect on liposome: An ESR study
Grammenos, Angeliki; Bahri, Mohamed Ali; Guelluy, Pierre-Henri et al.
2009In Biochemical and Biophysical Research Communications, 390, p. 5-9
Peer reviewed
 

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Keywords :
ESR; Microviscosity; Membrane; Cyclodextrin; Cholesterol; Liposome
Abstract :
[en] In the present work, the effect of Randomly-methylated-b-cyclodextrin (Rameb) on the microviscosity of dimyristoyl-L-a phosphatidylcholine (DMPC) bilayer was investigated using the electron spin resonance (ESR) technique. The ability of Rameb to extract membrane cholesterol was demonstrated. For the first time, the percentage of cholesterol extracted by Rameb from cholesterol doped DMPC bilayer was monitored and quantified throughout a wide Rameb concentration range. The effect of cholesterol on the inner part of the membrane was also investigated using 16-doxyl stearic acid spin label (16-DSA). 16-DSA seems to explore two different membrane domains and report their respective microviscosities. ESR experiments also establish that the presence of 30% of cholesterol in DMPC liposomes suppresses the jump in membrane fluidity at lipids phase-transition temperature (23.9°C).
Disciplines :
Physics
Biochemistry, biophysics & molecular biology
Author, co-author :
Grammenos, Angeliki ;  Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Bahri, Mohamed Ali  ;  Université de Liège - ULiège > Centre de recherches du cyclotron
Guelluy, Pierre-Henri ;  Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Piel, Géraldine ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Hoebeke, Maryse  ;  Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Language :
English
Title :
Quantification of Randomly-methylated-b-cyclodextrin effect on liposome: An ESR study
Publication date :
2009
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Academic Press, San Diego, United States - California
Volume :
390
Pages :
5-9
Peer reviewed :
Peer reviewed
Available on ORBi :
since 16 December 2009

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