Reference : Quantification of Randomly-methylated-b-cyclodextrin effect on liposome: An ESR study
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Physics
http://hdl.handle.net/2268/32812
Quantification of Randomly-methylated-b-cyclodextrin effect on liposome: An ESR study
English
Grammenos, Angeliki mailto [Université de Liège - ULg > Département de physique > Spectroscopie biomédicale >]
Bahri, Mohamed Ali mailto [Université de Liège - ULg > > Centre de recherches du cyclotron >]
Guelluy, Pierre-Henri mailto [Université de Liège - ULg > Département de physique > Spectroscopie biomédicale >]
Piel, Géraldine mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Hoebeke, Maryse mailto [Université de Liège - ULg > Département de physique > Spectroscopie biomédicale >]
2009
Biochemical and Biophysical Research Communications
Academic Press
390
5-9
Yes (verified by ORBi)
International
0006-291X
1090-2104
San Diego
CA
[en] ESR ; Microviscosity ; Membrane ; Cyclodextrin ; Cholesterol ; Liposome
[en] In the present work, the effect of Randomly-methylated-b-cyclodextrin (Rameb) on the microviscosity of dimyristoyl-L-a phosphatidylcholine (DMPC) bilayer was investigated using the electron spin resonance (ESR) technique. The ability of Rameb to extract membrane cholesterol was demonstrated. For the first time, the percentage of cholesterol extracted by Rameb from cholesterol doped DMPC bilayer was monitored and quantified throughout a wide Rameb concentration range. The effect of cholesterol on the inner part of the membrane was also investigated using 16-doxyl stearic acid spin label (16-DSA). 16-DSA seems to explore two different membrane domains and report their respective microviscosities. ESR experiments also establish that the presence of 30% of cholesterol in DMPC liposomes suppresses the jump in membrane fluidity at lipids phase-transition temperature (23.9°C).
Researchers ; Professionals
http://hdl.handle.net/2268/32812

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