Reference : Varicella-zoster virus IE63 protein represses the basal transcription machinery by di...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/3262
Varicella-zoster virus IE63 protein represses the basal transcription machinery by disorganizing the pre-initiation complex
English
Di Valentin, Emmanuel mailto [Université de Liège - ULg > Département des sciences de la vie > Virologie - Immunologie >]
Bontems, Sébastien mailto [Université de Liège - ULg > > Virologie - Immunologie >]
Habran, Lionel mailto [Centre Hospitalier Universitaire de Liège - CHU > > PLAN COS >]
Jolois, Olivier mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Markine-Goriaynoff, Nicolas [Université de Liège - ULg > > Immunologie et vaccinologie >]
Vanderplasschen, Alain mailto [Université de Liège - ULg > Département des sciences de la vie > Virologie et immunologie - GIGA-M : Coordination scientifique >]
Sadzot, Catherine mailto [Université de Liège - ULg > Département des sciences de la vie > Virologie - Immunologie >]
Piette, Jacques mailto [> > > >]
Mar-2005
Biological Chemistry
Walter De Gruyter & Co
386
3
255-267
Yes (verified by ORBi)
International
1431-6730
Berlin
[en] down-regulation ; general transcription factors ; repression ; transcription ; varicella-zoster
[en] Using transient transfection assays, regulation properties of varicella-zoster virus (VZV)-encoded IE63 protein were analyzed on several VZV immediate early (ORF4), early (ORF28) and late (ORF67) promoters. IE63 was shown to repress the basal activity of most of the promoters tested in epithelial (Vero) and neuronal (ND7) cells to various extents. Trans -repressing activities were also observed on heterologous viral and cellular promoters. Since a construct carrying only a TATA box sequence and a series of wild-type or mutated interleukin (IL)-8 promoters was also repressed by IE63, the role of upstream regulatory elements was ruled out. Importantly, the basal activity of a TATA-less promoter was not affected by IE63. Using a series of IE63 deletion constructs, amino acids 151-213 were shown to be essential to the transrepressing activity in Vero cells, while in ND7 cells the essential region extended to a much larger carboxy-terminal part of the protein. We also demonstrate that IE63 is capable of disrupting the transcriptional pre-initiation complex and of interacting with several general transcription factors. The central and carboxy-terminal domains of IE63 are important for these effects. Altogether, these results demonstrate that IE63 protein is a transcriptional repressor whose activity is directed towards general transcription factors.
http://hdl.handle.net/2268/3262
also: http://hdl.handle.net/2268/29351

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
BiolChem_DiValentin2005.pdfNo commentaryAuthor preprint1.79 MBView/Open
Restricted access
Di Valentin 2005.pdfNo commentaryPublisher postprint426.37 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.