[en] The aim of this work was to investigate whether the Penh index, measured using whoie body barometric piethysmography, can be used as a screening parameter to evaluate the airway reactivity and the intensity of the pulmonary response to endotoxins. Penh was firstly recorded in non-sedated freeiy moving piglets exposed (1) to a nebulized acetylchoiine (Ach) pre-treated or not with cienbuterol, or (2) to endotoxin challenge. To measure Penh simultaneously with total pulmonary resistance (RL), dynamic compliance (cd1) and intrapleural pressure changes (MaxPpl), an oesophageal balloon catheter technique was used and the piglets were anaesthetised. The recordings were performed during (1) an intravenous metacholine (Mch) challenge and (2) in endotoxin-exposed animais. In freely moving animais, Ach induced a significant dose-dependent increase in Penh, which was significantly blocked by clenbuterol. Endotoxin instillation aiso resulted in a significant rise in Penh while the corresponding response measured under anaesthesia was signiflcantly and positively correlated with RL and MaxzPpi. Similar results were obtained during Mch challenge but the Penh was negatively correlated with Cd. We conclude that Penh could be used in freely moving piglets as a screening index for airway reactivity and pulmonary functional changes in cholinergic and endotoxin challenges
Barnes P.J. Basbaum C.B. Nadel J.A. Autoradiographic localization of autonomic receptors in airway smooth muscle. Marked differences between large and small airways American Review of Respiratory Disease 127 1983 758-762
Chong B.T. Agrawal D.K. Romero F.A. Townley R.G. Measurement of bronchoconstriction using whole-body plethysmograph: comparison of freely moving versus restrained guinea pigs Journal of Pharmacology and Toxicology Methods 39 1998 163-168
Claridge J.A. Enelow R.I. Young J.S. Hemorrhage and resuscitation induce delayed inflammation and pulmonary dysfunction in mice Journal of Surgery Research 92 2000 206-213
Delaunois A. Segura P. Montano L.M. Vargas M.H. Ansay M. Gustin P. Comparison of ozone-induced effects on lung mechanics and hemodynamics in the rabbit Toxicology and Applied Pharmacology 150 1998 58-67
Djuric V.J. Cox G. Overstreet D.H. Smith L. Dragomir A. Steiner M. Genetically transmitted cholinergic hyperresponsiveness predisposes to experimental asthma Brain Behaviour and Immunity 12 1998 272-284
Gerbino A.J. Glenny R.W. Lung albumin accumulation is spatially heterogeneous but not correlated with regional pulmonary perfusion Journal of Applied Physiology 92 2002 279-287
Gustin P. Lekeux P. Lomba F. Clercx C. Mechanical properties of excised calf lungs: effects of airway obstruction with beads Research in Veterinary Science 42 1987 277-279
Gustin P. Lomba F. Bakima J. Lekeux P. van de Woestijne K.P. Partitioning of pulmonary resistance in calves Journal of Applied Physiology 62 1987 1826-1831
Hamelmann E. Schwarze J. Takeda K. Oshiba A. Larsen G.L. Irvin C.G. Gelfand E.W. Noninvasive measurement of airway responsiveness in allergic mice using barometric plethysmography American Journal of Respiratory and Critical Care Medicine 156 1997 766-775
Hoffman A.M. Dhupa N. Cimetti L. Airway reactivity measured by barometric whole-body plethysmography in healthy cats American Journal of Veterinary Research 60 1999 1487-1492
Intraraksa Y. Engen R.L. Switzer W.P. Pulmonary and hematologic changes in swine with Mycoplasma hyopneumoniae pneumonia American Journal of Veterinary Research 45 1984 474-477
Jang A.S. Choi I.S. Koh Y.I. Park C.S. Lee J.S. The relationship between alveolar epithelial proliferation and airway obstruction after ozone exposure Allergy 57 2002 737-740
Klein C. Reinhold P. Analysis of respiratory mechanics by impulse oscillometry in non- sedated and diazepam-sedated swine Research in Veterinary Science 70 2001 181-189
Krane C.M. Fortner C.N. Hand A.R. McGraw D.W. Lorenz J.N. Wert S.E. Towne J.E. Paul R.J. Whitsett J.A. Menon A.G. Aquaporin 5-deficient mouse lungs are hyperresponsive to cholinergic stimulation Proceedings of the National Academy of Science of USA 98 2001 14114-14119
Krombach F. Fiehl E. Burkhardt D. Rienmuller R. Konig G. Adelmann-Grill B.C. Idel H. Rosenbruch M. Short-term and long-term effects of serial bronchoalveolar lavages in a nonhuman primate model American Journal of Respiratory and Critical Care Medicine 150 1994 153-158
Maurenbrecher H. Lamy M. Deby-Dupont G. Frascarolo P. Hedenstierna G. An animal model of response and nonresponse to inhaled nitric oxide in endotoxin-induced lung injury Chest 120 2001 573-581
McFawn P.K. Gray P.R. Thomas J. Mitchell H.W. Pulmonary inflammation without bronchial hyperresponsiveness in vivo or in vitro after sephadex instillation in pigs Clinical and Experimental Pharmacology and Physiology 26 1999 105-108
Mitchell H.W. Cvetkovski R. Sparrow M.P. Gray P.R. McFawn P.K. Concurrent measurement of smooth muscle shortening, lumen narrowing and flow to acetylcholine in large and small porcine bronchi European Respiratory Journal 12 1998 1053-1061
O'Grady N.P. Preas H.L. Pugin J. Fiuza C. Tropea M. Reda D. Banks S.M. Suffredini A.F. Local inflammatory responses following bronchial endotoxin instillation in humans American Journal of Respiratory and Critical Care Medicine 163 2001 1591-1598
Padrid P. Snook S. Finucane T. Shiue P. Cozzi P. Solway J. Leff A.R. Persistent airway hyperresponsiveness and histologic alterations after chronic antigen challenge in cats American Journal of Respiratory and Critical Care Medicine 151 1995 184-193
Sciuto A.M. Lee R.B. Forster J.S. Cascio M.B. Clapp D.L. Moran T.S. Temporal changes in respiratory dynamics in mice exposed to phosgene Inhalation Toxicology 14 2002 487-501
Urbain B. Gustin P. Ansay M. Endotoxin-induced microvascular injury in isolated and perfused pig lungs Veterinary Research Communication 16 1992 453-464
Urbain B. Prouvost J. Beerens D. Ansay M. Gustin P. Acute effects of endotoxin inhalation on the respiratory tract in pigs : interaction with ammonia Inhalation Toxicology 8 1996 947-968
Vincke G. Danhaive P. Stiernet F. Peck M. Gustin P. Pulmonary inflammatory response of rats exposed to repeated nebulizations of cadmium American Journal of Respiratory and Critical Care Medicine 165 2002 A48
Wu Z.X. Zhou D. Chen G. Lee L.Y. Airway hyperresponsiveness to cigarette smoke in ovalbumin-sensitized guinea pigs American Journal of Respiratory and Critical Care Medicine 161 2000 73-80
Zeldin D.C. Wohlford-Lenane C. Chulada P. Bradbury J.A. Scarborough P.E. Roggli V. Langenbach R. Schwartz D.A. Airway inflammation and responsiveness in prostaglandin H synthase-deficient mice exposed to bacterial lipopolysaccharide American Journal of Respiratory and Cell Molecular Biology 25 2001 457-465