Reference : Involvement of polyamines in the adventitious rooting of micropropagated shoots of th...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Life sciences : Biotechnology
http://hdl.handle.net/2268/3248
Involvement of polyamines in the adventitious rooting of micropropagated shoots of the apple rootstock MM106
English
Naija, Sélima [Faculty of Sciences of Tunis > > Plant Biology and Biotechnology > >]
Elloumi, Nadhra [National Institute of Agronomic Research of Tunisia > > Biotechnology > >]
Ammar, Saida [Faculty of Sciences of Tunis > > Plant Biology and Biotechnology > >]
Kevers, Claire mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
Dommes, Jacques mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
2009
In Vitro Cellular & Developmental Biology-Plant
Springer Science & Business Media B.V.
45
83-91
Yes (verified by ORBi)
International
1054-5476
Wallingford
United Kingdom
[en] Auxin ; Inhibitors of polyamine metabolism ; micropropagation ; Peroxidase ; physiological phases
[en] Apple rootstock MM106 shoots, raised in vitro, rooted at 96.7% after culture on a medium supplemented with an auxin for 5 d in darkness followed by culture on a second medium without growth regulators for 25 d in light. In control conditions (in absence of auxin in the first medium), these shoots did not root. Putrescine (PUT), spermidine (SPD), cyclohexylamine (CHA), and aminoguanidine (AG) enhanced rooting when applied during the first d of culture in the absence of IBA; on the contrary, α-difluoromethylornithine (DFMO) added to the first medium with IBA inhibited rooting. The endogenous levels of indole 3-acetic acid (IAA) and indole 3-acetylaspartic acid (IAAsp) increased up to a maximum concentration at days 2 and 3, respectively, in initial rooting conditions. PUT, when added with IBA, did not affect the typical IAA and IAAsp increase; when applied alone, it provoked an increase of their levels. Similar results were recorded with CHA. SPD, AG, and DFMO did not induce an increase of IAA and IAAsp in nonrooting conditions. The levels of endogenous PUT increased to a maximum at day 2 in rooting conditions; it was slightly affected by exogenous PUT and CHA application but reduced by SPD, AG, and DFMO. In rooting conditions, if the first medium was supplemented with SPD or AG, a small increase in peroxidase activity was observed, similar to that obtained with PUT treatment. The present work indicates an involvement of polyamines in the control of rooting and an interaction with auxins during the physiological phase of rooting. The consequence of this relationship was a different rooting expression, according especially to the content of these regulators in the culture medium.
Researchers ; Professionals
http://hdl.handle.net/2268/3248
10.1007/s11627-008-9165-7

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