ORBi: Tabart Jessica - Comparative antioxidant capacities of phenolic compounds measured by various tests

Reference : Comparative antioxidant capacities of phenolic compounds measured by various tests


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Phytobiology (plant sciences, forestry, mycology...) Life sciences : Biotechnology
	To cite this reference:	http://hdl.handle.net/2268/3247

Title :	Comparative antioxidant capacities of phenolic compounds measured by various tests
Language :	English
Author, co-author :	Tabart, Jessica [Université de Liège - ULg > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
	Kevers, Claire [Université de Liège - ULg > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
	Pincemail, Joël [Centre Hospitalier Universitaire de Liège - CHU > > Chirurgie cardio-vasculaire >]
	Defraigne, Jean-Olivier [Université de Liège - ULg > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique >]
	Dommes, Jacques [Université de Liège - ULg > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
Publication date :	2009
Journal title :	Food Chemistry
Publisher :	Elsevier Science
Volume :	113
Pages :	1226-1233
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0308-8146
City :	Oxford
Country :	United Kingdom
Keywords :	[en] Antioxidant ; Anthocyanins ; Flavan-3-ols ; Flavonols ; Phenolics
Abstract :	[en] The purpose of this study was to compare the antioxidant capacities of standard compounds (phenolic compounds, ascorbic acid, and glutathione) as measured by various assays. Five methods were selected so as to span a diversity of technical approaches: TEAC (radical 2,2′-azino-bis(3-ethylbenzothiazoline)-6 sulphonic acid), DPPH (radical 2,2-diphenyl-1-picryhydrazyl used to measure reducing capacity), ORAC (oxygen radical scavenging capacity), red blood cell haemolysis (protection of biological sample), and ESR (electron spin resonance for direct free radical evaluation). Most compounds showed significant differences in free radical scavenging activity according to the method used. Of the 25 tested compounds, only a few, such as myricetin and gallocatechin, gave comparable activities in the various tests. To standardise reporting on antioxidant capacity, it is proposed to use a weighted mean of the values obtained using the DPPH, ORAC, resistance to haemolysis, and ESR assays. This strategy was used to test the antioxidant capacity of several beverages. The highest antioxidant capacity was observed for red wine, followed by green tea, orange juice, grape juice, vegetable juice, and apple juice.
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/3247
DOI :	10.1016/j.foodchem.2008.08.013

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