Reference : Counterdiffusion protein crystallisation in microgravity and its observation with Pro...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/32258
Counterdiffusion protein crystallisation in microgravity and its observation with PromISS (Protein Microscope for the International Space Station)
English
Zegers, Ingrid [Vrije Universiteit Brussel - VUB > Department Ultrastructure > > >]
Carotenuto, Luigi [MARS Center, Napoli > > > >]
Evrard, Christine mailto [Université Catholique de Louvain - UCL > Département de Chimie > Unité de Chimie Structurale (CSTR) > > >]
Garcia-Ruiz, JuanMa [Edifício BIC Granada > > > > >]
De Gieter, Philippe [Université Libre de Bruxelles - ULB > Microgravity Research centre > > > >]
Gonzales- Ramires, Luis [Edifício BIC Granada > > > > >]
Istasse, Eric [Université Libre de Bruxelles - ULB > Microgravity Research centre > > > >]
Legros, Jean-Claude [Université Libre de Bruxelles - ULB > Microgravity Research Centre > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Minetti, Christophe [Université Libre de Bruxelles - ULB > Microgravity Research centre > > > >]
Otalora, Fermin [Edifício BIC Granada > > > > >]
Queeckers, Patrick [Université Libre de Bruxelles - ULB > Microgravity Research centre > > > >]
Schockeart, Cédric [Université Libre de Bruxelles - ULB > Microgravity Research centre > > > >]
Van de Weerdt, Cécile mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Willaert, Ronnie [Vrije Universiteit Brussel - VUB > Department Ultrastructure > > > >]
Wyns, Lode [Vrije Universiteit Brussel - VUB > Department Ultrastructure > > > >]
Yourassowsky, Catherine [Université Libre de Bruxelles - ULB > Microgravity Research centre > > > >]
Dubois, Frank [Université Libre de Bruxelles - ULB > Microgravity Research centre > > > >]
2006
Microgravity Science and Technology
XVIII
165-169
Yes (verified by ORBi)
International
0938-0108
[en] protein ; crystallisation ; microgravity
[en] The crystallisation by counterdiffusion is a very efficient technique for obtaining high-quality protein crystals. A prerequisite for the use of counterdiffusion techniques is that mass transport must be controlled by diffusion alone. Sedimentation and convection can be avoided by either working in gelled systems, working in systems of small dimensions, or in the absence of gravity. We present the results from experiments performed on the ISS using the Protein Microscope for the International Space Station (PromISS), using digital holography to visualise crystal growth processes. We extensively characterised three model proteins for these experiments (cablys3*lysozyme, triose phosphate isomerase, and parvalbumin) and used these to assess the ISS as an environment for crystallisation by counterdiffusion. The possibility to visualise growth and movement of crystals in different types of experiments (capillary counterdiffusion and batch-type) is important, as movement of crystals is clearly not negligible.
http://hdl.handle.net/2268/32258

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