Reference : Anatomical and biochemical changes during adventitious rooting of apple rootstocks MM 10...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/2268/3223
Anatomical and biochemical changes during adventitious rooting of apple rootstocks MM 106 cultured in vitro.
English
Naija, Sélima [Faculty of Sciences of Tunis > > Plant Biology and Biotechnology > >]
Elloumi, Nadhra [National Institute of Agronomic Research of Tunisia > > > > Biotechnology > >]
Jbir, Najoua [Faculty of Sciences of Tunis > > > > Plant Biology and Biotechnology > >]
Ammar, Saida [Faculty of Sciences of Tunis > > > > Plant Biology and Biotechnology > >]
Kevers, Claire mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
May-2008
Comptes Rendus Biologies
Elsevier
331
7
518-25
Yes (verified by ORBi)
International
1631-0691
Paris
France
Adventitious roots ; Anatomical study ; Biochemical marker
[en] Adventitious rooting in microcuttings of Malus rootstocks MM106 was studied as regards their histological and biochemical aspects. Microcuttings from shoots raised in Murashige and Skoog's (1962) medium were transferred into a rooting medium containing IBA in the dark, then fixed 0, 3, 5, 7 and 10 days after. Some cambial zone and adjacent phloem cells became dense cytoplasm, nuclei with prominent nucleoli and the first cell divisions were observed at day 3. Meristemoids became individualized, consisting of densely staining cells (with enlarged nucleoli) formed outside the xylem by day 5. Identifiable root primordia with a conical shape and several cell layers were present at day 7. Roots with organized tissue system emerged from the stem 10 days after the root induction treatment. From these histological observations, it can be established that the rooting induction stage ended before day 3. The initiation stage, with the first histological modifications to the formation of meristemoids, would correspond to the transient increase of our biochemical marker (peroxidase activity) until day 5. The best rooting percentage obtained with cultures in the presence of auxin during 5 days confirms this hypothesis. The expression of rooting can then take place.
Researchers
http://hdl.handle.net/2268/3223
10.1016/j.crvi.2008.04.002

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