Article (Scientific journals)
Chiral Separation of Basic Drugs by Capillary Zone Electrophoresis with Cyclodextrin Additives
Bechet, I.; Paques, P.; Fillet, Marianne et al.
1994In Electrophoresis, 15 (6), p. 818-23
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Abstract :
[en] The enantiomers of a series of basic drugs were separated in capillary zone electrophoresis (CZE) in phosphate buffers, pH 3, containing beta-cyclodextrin or one of its derivatives as chiral selectors and uncoated fused silica capillaries thermostated at 15 degrees C. The nature of the cationic component of the background electrolyte was found to have a significant influence on achiral resolution and peak symmetry. The best results were obtained with triethanolamine, which was then used to adjust the buffer pH in all further experiments. The effects on chiral resolution of the nature and concentration of cyclodextrin, of the addition of methanol, and of capillary temperature were studied. Maximum resolution was obtained at a particular cyclodextrin concentration for each analyte, depending on the affinity of the analyte for this cyclodextrin. On the basis of the results, the effects of methanol addition and temperature on enantiomeric resolution could be explained and predicted. Numerous chiral separations are presented and suggestions for the rapid optimization of CZE enantioseparations with cyclodextrin additives are given.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Bechet, I.
Paques, P.
Fillet, Marianne ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Hubert, Philippe  ;  Université de Liège - ULiège > Chimie analytique
Crommen, Jacques ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Chiral Separation of Basic Drugs by Capillary Zone Electrophoresis with Cyclodextrin Additives
Publication date :
June 1994
Journal title :
Electrophoresis
ISSN :
0173-0835
eISSN :
1522-2683
Publisher :
John Wiley & Sons, United Kingdom
Volume :
15
Issue :
6
Pages :
818-23
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 May 2010

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