Reference : Effect of pH on glucose and starch fermentation in batch and sequenced-batch mode with a...
Scientific journals : Article
Life sciences : Biotechnology
Life sciences : Microbiology
http://hdl.handle.net/2268/31791
Effect of pH on glucose and starch fermentation in batch and sequenced-batch mode with a recently isolated strain of hydrogen-producing Clostridium butyricum CWBI1009
English
Masset, Julien mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Hiligsmann, Serge mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Hamilton, Christopher [Université de Liège - ULg > > Centre Wallon de biologie industrielle >]
Beckers, Laurent mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Franck, Fabrice mailto [Université de Liège - ULg > > Biochimie végétale >]
Thonart, Philippe mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles - Bio-industries >]
Apr-2010
International Journal of Hydrogen Energy
Pergamon Press - An Imprint of Elsevier Science
35
8
3371-3378
Yes (verified by ORBi)
International
0360-3199
[en] Clostridium butyricum ; starch ; hydrogen production ; pH ; batch ; sequenced-batch
[en] This paper reports investigations carried out to determine the optimum culture conditions for
the production of hydrogen with a recently isolated strain Clostridium butyricum CWBI1009.
The production rates and yields were investigated at 30 °C in a 2.3 l bioreactor operated in
batch and sequenced-batch mode using glucose and starch as substrates. In order to study the
precise effect of a stable pH on hydrogen production, and the metabolite pathway involved,
cultures were conducted with pH controlled at different levels ranging from 4.7 to 7.3
(maximum range of 0.15 pH unit around the pH level). For glucose the maximum yield (1.7
mol H2 mol-1 glucose) was measured when the pH was maintained at 5.2. The acetate and
butyrate yields were 0.35 mol acetate mol-1 glucose and 0.6 mol butyrate mol-1 glucose. For
starch a maximum yield of 2.0 mol H2 mol-1 hexose, and a maximum production rate of 15
mol H2 mol-1 hexose h-1 were obtained at pH 5.6 when the acetate and butyrate yields were
0.47 mol acetate mol-1 hexose and 0.67 mol butyrate mol-1 hexose.
Centre Wallon de Biologie Industrielle ; Laboratoire de biochimie végétale
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Action de Recherches concertées ARC 07/12 04- ULg- Communauté française ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
Etude de la production d'hydrogène par les bactéries anaérobies chimiotrophes (dark-fermentation)
Researchers ; Professionals ; Students ; General public
http://hdl.handle.net/2268/31791
10.1016/j.ijhydene.2010.01.061
http://cwbi.fsagx.ac.be/
http://www.microh2.ulg.ac.be

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