ORBi: Masset Julien - Effect of pH on glucose and starch fermentation in batch and sequenced-batch mode with a recently isolated strain of hydrogen-producing Clostridium butyricum CWBI1009

Reference : Effect of pH on glucose and starch fermentation in batch and sequenced-batch mode with a...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Microbiology Life sciences : Biotechnology
	To cite this reference:	http://hdl.handle.net/2268/31791

Title :	Effect of pH on glucose and starch fermentation in batch and sequenced-batch mode with a recently isolated strain of hydrogen-producing Clostridium butyricum CWBI1009
Language :	English
Author, co-author :	Masset, Julien [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
	Hiligsmann, Serge [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
	Hamilton, Christopher [Université de Liège - ULg > > Centre Wallon de biologie industrielle >]
	Beckers, Laurent [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
	Franck, Fabrice [Université de Liège - ULg > > Biochimie végétale >]
	Thonart, Philippe [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles - Bio-industries >]
Publication date :	Apr-2010
Journal title :	International Journal of Hydrogen Energy
Publisher :	Pergamon Press - An Imprint of Elsevier Science
Volume :	35
Issue/season :	8
Pages :	3371-3378
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0360-3199
Keywords :	[en] Clostridium butyricum ; starch ; hydrogen production ; pH ; batch ; sequenced-batch
Abstract :	[en] This paper reports investigations carried out to determine the optimum culture conditions for the production of hydrogen with a recently isolated strain Clostridium butyricum CWBI1009. The production rates and yields were investigated at 30 °C in a 2.3 l bioreactor operated in batch and sequenced-batch mode using glucose and starch as substrates. In order to study the precise effect of a stable pH on hydrogen production, and the metabolite pathway involved, cultures were conducted with pH controlled at different levels ranging from 4.7 to 7.3 (maximum range of 0.15 pH unit around the pH level). For glucose the maximum yield (1.7 mol H2 mol-1 glucose) was measured when the pH was maintained at 5.2. The acetate and butyrate yields were 0.35 mol acetate mol-1 glucose and 0.6 mol butyrate mol-1 glucose. For starch a maximum yield of 2.0 mol H2 mol-1 hexose, and a maximum production rate of 15 mol H2 mol-1 hexose h-1 were obtained at pH 5.6 when the acetate and butyrate yields were 0.47 mol acetate mol-1 hexose and 0.67 mol butyrate mol-1 hexose.
Research units :	Centre Wallon de Biologie Industrielle ; Laboratoire de biochimie végétale
Funders :	Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Action de Recherches concertées ARC 07/12 04- ULg- Communauté française ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
Name of the research project :	Etude de la production d'hydrogène par les bactéries anaérobies chimiotrophes (dark-fermentation)
Target :	Researchers ; Professionals ; Students ; General public
Permalink :	http://hdl.handle.net/2268/31791
DOI :	10.1016/j.ijhydene.2010.01.061
Other URL :	http://cwbi.fsagx.ac.be/
	http://www.microh2.ulg.ac.be

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Open access	Masset et al. 2010_IJHE_H2 produc° Clostridium but_ppa.pdf		Author postprint	517.5 kB	View/Open

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