Reference : The 16-kilodalton N-terminal fragment of human prolactin is a potent inhibitor of angiog...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/31668
The 16-kilodalton N-terminal fragment of human prolactin is a potent inhibitor of angiogenesis
English
Clapp, Carmen [> > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Guzman, Raphaël C [> > > >]
Rentier-Delrue, Françoise mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie et génétique moléculaire - GIGA-R : Coordination scientifique >]
Weiner, Richard I [> > > >]
1993
Endocrinology
Endocrine Society
133
3
1292-9
Yes (verified by ORBi)
0013-7227
Chevy Chase
MD
[en] Allantois/blood supply ; Animals ; Brain/blood supply ; Capillaries/cytology ; Cattle ; Cell Division/drug effects ; Cells, Cultured ; Chick Embryo ; Chorion/blood supply ; Collagen ; Culture Media ; Endothelium, Vascular/cytology ; Endotoxins/analysis ; Epithelial Cells ; Epithelium/drug effects ; Female ; Fibroblast Growth Factor 2/pharmacology ; Humans ; Mammary Glands, Animal/cytology/drug effects ; *Neovascularization, Pathologic/drug therapy ; Peptide Fragments/*pharmacology/therapeutic use ; Prolactin/*pharmacology/therapeutic use ; Protein-Tyrosine Kinases/pharmacology ; Rats ; Rats, Inbred Lew ; Receptors, Vascular Endothelial Growth Factor ; Recombinant Proteins/pharmacology/therapeutic use ; Umbilical Veins/cytology
[en] The formation of a new blood supply, angiogenesis, is an essential component of carcinogenesis and unrestricted tumor growth. A substance capable of inhibiting angiogenesis would be of considerable therapeutic potential in the treatment of cancer. We previously reported that the 16-kilodalton N-terminal fragment of rat PRL (16K rPRL) was a potent inhibitor of capillary endothelial cell proliferation via a novel receptor. We now report that the nanomolar concentrations of recombinant human 16K PRL inhibit basal and basic fibroblast growth factor- or vascular endothelial growth factor-stimulated growth of bovine brain capillary endothelial cells. 16K human (h) PRL also inhibits stimulation of human umbilical vein endothelial cell proliferation by basic fibroblast growth factor. The organization of endothelial cells into capillary-like structures in type I collagen gels is also prevented by 16K hPRL. Furthermore, in an in vivo assay, the chick embryo chorioallantoic membrane assay, 16K hPRL as well as 16K rPRL were potent inhibitors of capillary formation. 16K hPRL, like 16K rPRL, maintains its biological activity as a partial PRL agonist at PRL receptors on mammary gland epithelial cells. These data demonstrate for the first time that the biological activity of 16K rPRL is not unique and that similar fragments of hPRL are active. The antiangiogenic activity of these molecules is conserved across avian and mammalian species. That 16K hPRL is a potent antiangiogenic factor in in vitro and an in vivo assay raises the exciting potential of this peptide being capable of inhibiting tumor growth.
http://hdl.handle.net/2268/31668
http://endo.endojournals.org/cgi/reprint/133/3/1292
1993/09/01
http://endo.endojournals.org

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