Article (Scientific journals)
Characterization of a single strong tissue-specific enhancer downstream from the three human genes encoding placental lactogen
Jacquemin, Patrick; Oury, Cécile; Peers, Bernard et al.
1994In Molecular and Cellular Biology, 14 (1), p. 93-103
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Keywords :
Base Sequence; Binding Sites/genetics; DNA/genetics; DNA-Binding Proteins/metabolism; *Enhancer Elements, Genetic; Female; Gene Expression Regulation; Growth Hormone/genetics; Hela Cells; Humans; Models, Genetic; Molecular Sequence Data; Multigene Family; *Nuclear Proteins; Placenta/metabolism; Placental Lactogen/*genetics; Pregnancy; Protein Binding; Sequence Homology, Nucleic Acid; Tissue Distribution; Transcription Factors/metabolism
Abstract :
[en] The human genes coding for growth hormone (hGH) and placental lactogen (choriosomatomammotropic hormone [hCS]) are clustered on chromosome 17 in the following order: 5' hGH-N hCS-L hCS-A hGH-V hCS-B 3'. So far, a single placenta-specific enhancer has been identified in the locus, 2 kb downstream from the hCS-B gene, and shown to comprise one in vitro binding site for a nuclear protein. We here provide evidence that the hCS-B enhancer is more complex: (i) protection against DNase I digestion in the 3' flanking region of the hCS-B gene reveals four binding sites (DF-1, DF-2, DF-3, and DF-4) for nuclear proteins from either placental or HeLa cells, and (ii) placenta-specific enhancer activity can be fully exerted in transient expression experiments by a 126-bp fragment comprising the DF-3 and DF-4 protein-binding sites. By dissecting this region, we show that enhancer activity is mediated by a synergy between DF-3 and DF-4. Competitions with various oligonucleotides in footprinting and gel retardation experiments indicate that the same protein or set of proteins, different in HeLa and placenta cell nuclei, interacts with sites DF-2, DF-3, and DF-4. We also studied the regions of the hCS-L and hCS-A genes which are highly similar to the hCS-B enhancer. Although they each present the same four protein-binding sites, they exhibit only minor enhancer activity.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Jacquemin, Patrick
Oury, Cécile  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique générale et humaine
Peers, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Morin, Alexis ;  Centre Hospitalier Universitaire de Liège - CHU > Ateliers biomédicaux
Belayew, Alexandra
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Characterization of a single strong tissue-specific enhancer downstream from the three human genes encoding placental lactogen
Publication date :
1994
Journal title :
Molecular and Cellular Biology
ISSN :
0270-7306
eISSN :
1098-5549
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
Volume :
14
Issue :
1
Pages :
93-103
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 December 2009

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