Reference : Thyroid hormone inhibits the human prolactin gene promoter by interfering with activatin...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/31657
Thyroid hormone inhibits the human prolactin gene promoter by interfering with activating protein-1 and estrogen stimulations
English
Pernasetti, Flavia [Université de Liège - ULG > > > >]
Caccavelli, L. [Université de Liège - ULG > > > >]
Van de Weerdt, Cécile mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Muller, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
1997
Molecular Endocrinology (Baltimore, Md.)
Endocrine Society
11
7
986-96
Yes (verified by ORBi)
International
0888-8809
Chevy Chase
MD
[en] Animals ; Base Sequence ; Cell Line ; DNA-Binding Proteins/metabolism ; Estradiol/*pharmacology ; Forskolin/pharmacology ; Gene Expression/drug effects ; Hela Cells ; Humans ; Pituitary Gland/cytology/drug effects/*metabolism ; Prolactin/drug effects/*genetics ; Promoter Regions, Genetic/*drug effects/genetics ; Rats ; Sensitivity and Specificity ; Transcription Factor AP-1/drug effects/*genetics/metabolism ; Transcription Factor Pit-1 ; Transcription Factors/metabolism ; Triiodothyronine/*pharmacology
[en] Transcription of the human PRL (hPRL) gene in the pituitary is subject to tissue-specific and multihormonal regulation involving two main regulatory regions, a proximal promoter and a distal enhancer. In this report we show that thyroid hormone inhibits the expression of the hPRL gene in rat pituitary cells. Transient expression experiments show that thyroid hormone regulation involves a strong inhibitory element, located in the proximal (-164/-35) promoter, which is modulated by a more distal stimulatory response control region. Gel retardation experiments reveal that the thyroid hormone receptor does not bind to the proximal negative element. We show the existence of an activating protein-1 (AP-1) response element located at positions -61 to -54 of the proximal promoter, conferring AP-1 stimulation to the hPRL promoter. This AP-1 induction is abolished when hormone-bound thyroid hormone receptor is present, indicating that there is an interference between the thyroid hormone receptor and AP-1 regulatory pathways. Furthermore, using the complete hPRL upstream region, we show that estrogen induction is abolished by simultaneous thyroid hormone treatment.
http://hdl.handle.net/2268/31657
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3G-3RM6VK0-6&_user=532038&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000026659&_version=1&_urlVersion=0&_userid=532038&md5=821bf23156aa046426e8ee41a0fbd8e7#fn1
1997/06/01
http://endo.endojournals.org/

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