Reference : The glucocorticoid receptor inhibits the human prolactin gene expression by interfere...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/31656
The glucocorticoid receptor inhibits the human prolactin gene expression by interference with Pit-1 activity
English
Nalda, Asunción M [Université Paris XI > > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Muller, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
1997
Molecular & Cellular Endocrinology
North Holland Publishing Company
134
2
129-37
Yes (verified by ORBi)
International
0303-7207
Amsterdam
The Netherlands
[en] Animals ; COS Cells ; Cyclic AMP/metabolism ; DNA/metabolism ; DNA-Binding Proteins/drug effects/*metabolism/physiology ; *Gene Expression Regulation/drug effects ; Hela Cells ; Humans ; Prolactin/*antagonists & inhibitors/*genetics ; Promoter Regions, Genetic/drug effects ; Protein Binding/genetics ; Rats ; Receptors, Glucocorticoid/*physiology ; Transcription Factor Pit-1 ; Transcription Factors/drug effects/*metabolism/physiology ; Tumor Cells, Cultured
[en] Glucocorticoids have been shown to inhibit the activity of the human prolactin (hPRL) promoter. Using transient expression experiments in rat pituitary cells, we located the sequence conferring glucocorticoid inhibition to a region which contains Pit-1 binding sites, responsible for pituitary-specific expression, but does not seem to contain a glucocorticoid receptor (GR) binding site. Co-transfection experiments in non-pituitary cell lines, using expression vectors for Pit-1 and different mutants of the human GR show that inhibition of the hPRL gene is seen only in the presence of Pit-1 and GR, and that the DNA binding function of the receptor is not required. Immunoprecipitation studies show that either anti-GR or anti-Pit-1 antibodies are able to co-precipitate GR and Pit-1, suggesting an interaction between these factors. We conclude that the activated GR functionally interferes with the pituitary specific factor Pit-1, thereby leading to the observed transcriptional repression.
http://hdl.handle.net/2268/31656
10.1016/S0303-7207(97)00176-7
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3G-3RM6VK0-6&_user=532038&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000026659&_version=1&_urlVersion=0&_userid=532038&md5=821bf23156aa046426e8ee41a0fbd8e7#fn1
1998/01/13
www.elsevier.com

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