Reference : Pit-1 mediates cell-specific and cAMP-induced transcription of the tilapia GH gene
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/31648
Pit-1 mediates cell-specific and cAMP-induced transcription of the tilapia GH gene
English
Sekkali, B. [> > > >]
Belayew, A. [University of Leu6en > > > >]
Bortolussi, M. [Universita di Padova > > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Muller, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
1999
Molecular & Cellular Endocrinology
North Holland Publishing Company
152
1-2
111-23
Yes (verified by ORBi)
International
0303-7207
Amsterdam
The Netherlands
[en] Amino Acid Sequence ; Animals ; Base Sequence ; Cyclic AMP/*pharmacology ; DNA-Binding Proteins/*genetics/metabolism ; Gene Expression Regulation/*drug effects ; Growth Hormone/*genetics/metabolism ; Molecular Sequence Data ; Promoter Regions, Genetic/genetics ; Rats ; Sequence Alignment ; Tilapia/*genetics ; Transcription Factor Pit-1 ; Transcription Factors/*genetics/metabolism ; Transcription, Genetic/drug effects
[en] Expression of the tilapia growth hormone (tiGH) gene is pituitary-specific and controlled by intracellular cAMP levels. DNaseI protection experiments allowed us to identify four Pit-1 binding sites in the tiGH - 465/ + 19 region. Deletion and mutagenesis analysis revealed that the - 131/+ 19 region, containing two Pit-1 sites, or four copies of the most proximal site tiGHF1 fused to the heterologous Tk promoter, confer high level expression in rat pituitary cells and direct transcription in non-pituitary cells only after expression of rat Pit-1. We show that a tilapia pituitary factor specifically binds to site tiGHF1 and obtained a partial cDNA sequence coding for tilapia Pit-1. The cAMP stimulation is mediated by the proximal (- 131/- 31) promoter region. It is Pit-1-dependent and requires the tiGHF1 site. In addition, four copies of this site confer cAMP inducibility to the Tk promoter in GC cells.
Applied and Fundamental FISH Research Center - AFFISH-RC ; Giga-Development and Stem Cells
http://hdl.handle.net/2268/31648
10.1016/S0303-7207(99)00053-2
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3G-3WMJSXW-D&_user=532038&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000026659&_version=1&_urlVersion=0&_userid=532038&md5=296dfad6863a1c387e9b52c0aa540fd2
1999/08/04
www.elsevier.com

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