Reference : Lys13 plays a crucial role in the functional adaptation of the thermophilic triose-ph...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/31644
Lys13 plays a crucial role in the functional adaptation of the thermophilic triose-phosphate isomerase from Bacillus stearothermophilus to high temperatures
English
Alvarez, Marco [ > > ]
Wouters, Johan [Facultés Universitaires Notre-Dame de la Paix > > > >]
Maes, Dominique [Vrije Universiteit Brussel > > > >]
Mainfroid, Véronique [> > > >]
Rentier-Delrue, Françoise mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie et génétique moléculaire - GIGA-R : Coordination scientifique >]
Wyns, Lode [Vrije Universiteit Brussel > > > >]
Depiereux, Eric [Facultés Universitaires Notre-Dame de la Paix > > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
1999
Journal of Biological Chemistry
American Society for Biochemistry and Molecular Biology
274
27
19181-7
Yes (verified by ORBi)
0021-9258
1083-351X
Baltimore
MD
[en] *Adaptation, Physiological ; Asparagine/metabolism ; Bacillus stearothermophilus ; Crystallography, X-Ray ; Enzyme Stability ; Glycine/metabolism ; *Hot Temperature ; Lysine/*metabolism ; Models, Molecular ; Molecular Sequence Data ; Protein Conformation ; Structure-Activity Relationship ; Triose-Phosphate Isomerase/*metabolism
[en] The thermophilic triose-phosphate isomerases (TIMs) of Bacillus stearothermophilus (bTIM) and Thermotoga maritima (tTIM) have been found to possess a His12-Lys13 pair instead of the Asn12-Gly13 pair normally present in mesophilic TIMs. His12 in bTIM was proposed to prevent deamidation at high temperature, while the precise role of Lys13 is unknown. To investigate the role of the His12 and Lys13 pair in the enzyme's thermoadaptation, we reintroduced the "mesophilic residues" Asn and Gly into both thermophilic TIMs. Neither double mutant displayed diminished structural stability, but the bTIM double mutant showed drastically reduced catalytic activity. No similar behavior was observed with the tTIM double mutant, suggesting that the presence of the His12 and Lys13 cannot be systematically correlated to thermoadaptation in TIMs. We determined the crystal structure of the bTIM double mutant complexed with 2-phosphoglycolate to 2.4-A resolution. A molecular dynamics simulation showed that upon substitution of Lys13 to Gly an increase of the flexibility of loop 1 is observed, causing an incorrect orientation of the catalytic Lys10. This suggests that Lys13 in bTIM plays a crucial role in the functional adaptation of this enzyme to high temperature. Analysis of bTIM single mutants supports this assumption.
http://hdl.handle.net/2268/31644
http://www.jbc.org/cgi/content/full/274/27/19181
1999/06/26

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