Reference : Sugar beet leaves as new source of hydroperoxide lyase in a bioprocess producing green-n...

	Document type :	Scientific journals : Article
	Discipline(s) :	Physical, chemical, mathematical & earth Sciences : Chemistry Life sciences : Agriculture & agronomy
	To cite this reference:	http://hdl.handle.net/2268/31554

Title :	Sugar beet leaves as new source of hydroperoxide lyase in a bioprocess producing green-note aldehydes.
Language :	English
Author, co-author :	Rabetafika, Holy-Nadia [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
	Gigot, Cédric [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
	Fauconnier, Marie-Laure [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
	Ongena, MARC [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
	Destain, Jacqueline [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
	Du Jardin, Patrick [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
	Wathelet, Jean-Paul [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
	Thonart, Philippe [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles >]
Publication date :	2008
Journal title :	Biotechnology Letters
Publisher :	Kluwer Academic Publishers
Volume :	30
Issue/season :	6
Pages :	1115-9
Audience :	International
ISSN :	0141-5492
City :	Dordrecht
Country :	The Netherlands
Keywords :	[en] Aldehyde-Lyases/isolation & purification/metabolism ; Aldehydes/metabolism ; Beta vulgaris/enzymology ; Chromatography, High Pressure Liquid ; Cytochrome P-450 Enzyme System/isolation & purification/metabolism ; Hexobarbital/metabolism ; Hydrogen-Ion Concentration ; Linoleic Acids/metabolism ; Linolenic Acids/metabolism ; Lipid Peroxides/metabolism ; Plant Leaves/enzymology
Abstract :	[en] Hydroperoxide lyase activity was found in sugar beet leaves. Its optimum pH and temperature were, respectively, 6.7 and 22 degrees C. Under these conditions, conversion of linolenic acid 13-hydroperoxide to cis-3-hexenal with a maximum yield of 80% was reached after only 2 min. The stability of cis-3-hexenal was improved by acidifying the reaction medium. Based on these studies, a bioprocess producing green-note aldehydes in a laboratory-scale was achieved.
Permalink :	http://hdl.handle.net/2268/31554
DOI :	10.1007/s10529-008-9652-2

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