Article (Scientific journals)
Systemic resistance and lipoxygenase-related defence response induced in tomato by Pseudomonas putida strain BTP1.
Akram, Adam; Ongena, MARC; Duby, Franceline et al.
2008In BMC Plant Biology, 8, p. 113
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Keywords :
Antifungal Agents/metabolism; Botrytis/pathogenicity; Chromatography, High Pressure Liquid; Gene Expression Regulation, Plant; Genes, Plant; Immunity, Innate; Lipoxygenase/genetics/metabolism; Lycopersicon esculentum/enzymology/genetics/microbiology; Mass Spectrometry; Plant Diseases/genetics/immunology; Plant Leaves/enzymology/genetics/microbiology; Plant Proteins/genetics/metabolism; Pseudomonas putida/physiology; RNA, Plant/genetics; Terpenes/metabolism
Abstract :
[en] BACKGROUND: Previous studies showed the ability of Pseudomonas putida strain BTP1 to promote induced systemic resistance (ISR) in different host plants. Since ISR is long-lasting and not conducive for development of resistance of the targeted pathogen, this phenomenon can take part of disease control strategies. However, in spite of the numerous examples of ISR induced by PGPR in plants, only a few biochemical studies have associated the protective effect with specific host metabolic changes. RESULTS: In this study, we showed the protective effect of this bacterium in tomato against Botrytis cinerea. Following treatment by P. putida BTP1, analyses of acid-hydrolyzed leaf extracts showed an accumulation of antifungal material after pathogen infection. The fungitoxic compounds thus mainly accumulate as conjugates from which active aglycones may be liberated through the activity of hydrolytic enzymes. These results suggest that strain BTP1 can elicit systemic phytoalexin accumulation in tomato as one defence mechanism. On another hand, we have shown that key enzymes of the lipoxygenase pathway are stimulated in plants treated with the bacteria as compared with control plants. Interestingly, this stimulation is observed only after pathogen challenge in agreement with the priming concept almost invariably associated with the ISR phenomenon. CONCLUSION: Through the demonstration of phytoalexin accumulation and LOX pathway stimulation in tomato, this work provides new insights into the diversity of defence mechanisms that are inducible by non-pathogenic bacteria in the context of ISR.
Disciplines :
Microbiology
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Akram, Adam
Ongena, MARC ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Duby, Franceline
Dommes, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Thonart, Philippe ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles
Language :
English
Title :
Systemic resistance and lipoxygenase-related defence response induced in tomato by Pseudomonas putida strain BTP1.
Publication date :
2008
Journal title :
BMC Plant Biology
eISSN :
1471-2229
Publisher :
BioMed Central
Volume :
8
Pages :
113
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 03 December 2009

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