Reference : Isolation of an n-alkylated benzylamine derivative from Pseudomonas putida BTP1 as el...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/31439
Isolation of an n-alkylated benzylamine derivative from Pseudomonas putida BTP1 as elicitor of induced systemic resistance in bean
English
Ongena, MARC mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Jourdan, Emmanuel [Université de Liège - ULg > > Centre Wallon de biologie industrielle >]
Schafer, M. [> > > >]
Kech, C. [> > > >]
Budzikiewicz, H. [> > > >]
Luxen, André mailto [Université de Liège - ULg > > Chimie organique de synthèse >]
Thonart, Philippe mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles >]
2005
Molecular Plant-Microbe Interactions
Amer Phytopathological Soc
18
6
562-569
Yes (verified by ORBi)
International
0894-0282
St Paul
[en] biological control ; plant growth-promoting rhizobacteria
[en] Root treatment of Phaseolus vulgaris with the nonpathogenic Pseudomonas putida BTP1 led to significant reduction of the disease caused by the pathogen Botrytis cinerea on leaves. The molecular determinant of P putida BTP1 mainly responsible for the induced systemic resistance (ISR) was isolated from cell-free culture fluid after growth of the strain in the iron-poor casamino acid medium. Mass spectrometry analyses performed on both the bacterial product and synthetic analogues revealed a polyalkylated benzylamine structure, with the quaternary ammonium substituted by methyl, ethyl, and C-13 aliphatic groups responsible for the relative hydrophobicity of the molecule. The specific involvement of the N-alkylated benzylamine derivative (NABD) in ISR elicitation was first evidenced by testing the purified compound that mimicked the protective effect afforded by crude supernatant samples. The evidence was supported by the loss of elicitor activity of mutants impaired in NABD biosynthesis. Our experiments also showed that other iron-regulated metabolites secreted by the strain are not involved in ISR stimulation. Thus, these results indicate a wider variety of Pseudomonas determinants for ISR than reported to date.
http://hdl.handle.net/2268/31439

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