Reference : Role of lipopeptides produced by Bacillus subtilis GA1 in the reduction of grey mould...
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biotechnology
http://hdl.handle.net/2268/31404
Role of lipopeptides produced by Bacillus subtilis GA1 in the reduction of grey mould disease caused by Botrytis cinerea on apple
English
Toure, Y. [> > > >]
Ongena, MARC mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Jacques, P. [> > > >]
Guiro, A. [> > > >]
Thonart, Philippe mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
2004
Journal of Applied Microbiology
Blackwell Publishing Ltd
96
5
1151-1160
Yes (verified by ORBi)
International
1364-5072
Oxford
[en] apple ; Bacillus subtilis ; biological control ; biopesticide ; fengycin ; grey mould ; lipopeptide
[en] AIM: Test of Bacillus subtilis strain GA1 for its potential to control grey mould disease of apple caused by Botrytis cinerea. METHODS AND RESULTS: GA1 was first tested for its ability to antagonize in vitro the growth of a wide variety of plant pathogenic fungi responsible for diseases of economical importance. The potential of strain GA1 to reduce post-harvest infection caused by B. cinerea was tested on apples by treating artificially wounded fruits with endospore suspensions. Strain GA1 was very effective at reducing disease incidence during the first 5 days following pathogen inoculation and a 80% protection level was maintained over the next 10 days. Treatment of fruits with an extract of GA1 culture supernatant also exerted a strong preventive effect on the development of grey mould. Further analysis of this extract revealed that strain GA1 produces a wide variety of antifungal lipopeptide isomers from the iturin, fengycin and surfactin families. A strong evidence for the involvement of such compounds in disease reduction arose from the recovery of fengycins from protected fruit sites colonized by bacterial cells. CONCLUSIONS: The results presented here demonstrate that, despite unfavourable pH, B. subtilis endospores inoculated on apple pulp can readily germinate allowing significant cell populations to establish and efficient in vivo synthesis of lipopeptides which could be related to grey mould reduction. SIGNIFICANCE AND IMPACT OF THE STUDY: This work enables for the first time to correlate the strong protective effect of a particular B. subtilis strain against grey mould with in situ production of fengycins in infected sites of apple fruits.
http://hdl.handle.net/2268/31404
10.1111/j.1365-2672.2004.02252.x

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
J Applied Micriobiol 2004.pdfPublisher postprint197.36 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.