Reference : Synthesis of peptide-modified pyoverdins by a fluorescent Pseudomonas strain grown in is...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/31334
Synthesis of peptide-modified pyoverdins by a fluorescent Pseudomonas strain grown in isoleucine-supplemented medium
English
Ongena, MARC mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Jacques, Philippe [> > > >]
De Pauw, Edwin mailto [Université de Liège - ULg > > Chimie physique, spectrométrie de masse >]
Thonart, Philippe mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles >]
2002
Letters in Peptide Science
Kluwer Academic Publ
8
1
21-27
Yes
International
0929-5666
Dordrecht
[en] electrospray ionization mass spectrometry ; iron acquisition ; non-ribosomal peptide synthetase ; Pseudomonas putida ; siderophore
[en] The pattern of pyoverdin-type siderophores produced by Pseudomonas putida BTP16 in iron-limited succinate medium supplemented or not with isoleucine was characterized by electrospray ionization mass spectrometry. Two new compounds were observed in culture supernatants from BTP16 grown in the presence of isoleucine. For both molecules, mass differences with pyoverdins originally synthesized by the strain were only interpreted considering the incorporation of Ile into the peptide chain. Further evidence supporting this phenomenon was provided by radioactivity incorporation in pyoverdins produced in the presence of C-14-labeled Ile. Our results are discussed in relation to the non-ribosomal mechanism supposed to be involved in the synthesis of these molecules.
http://hdl.handle.net/2268/31334

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Letters in Peptide Science 2002.pdfPublisher postprint106.8 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.