Reference : Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism's antagoni...
Scientific journals : Article
Life sciences : Biotechnology
Life sciences : Microbiology
http://hdl.handle.net/2268/31330
Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism's antagonistic and biocontrol activities
English
Leclère, Valérie [University of Science and Technology of Lille > Laboratory of Microbial Bioprocesses, Polytech’Lille >]
Béchet, Max [University of Science and Technology of Lille > Laboratory of Microbial Bioprocesses, Polytech’Lille >]
Adam, Akram [> >]
Guez, Jean-Sébastien [> > > >]
Wathelet, Bernard mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Ongena, Marc mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Thonart, Philippe mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Gancel, Frédérique [> > > >]
Chollet-Imbert, Marlène [> > > >]
Jacques, Philippe [> > > >]
Aug-2005
Applied and Environmental Microbiology
Amer Soc Microbiology
71
8
4577-4584
Yes (verified by ORBi)
International
0099-2240
Washington
[en] Bacillus subtilis ; mycosubtilin
[en] A Bacillus subtilis derivative was obtained from strain ATCC 6633 by replacement of the native promoter (if he mycosubtilin operon by a constitutive promoter originating from the replication gene repU of the Staphylococcus aureus plasmid pUB110. The recombinant strain, designated BBG100, produced up to 15-fold more mycosubtilin than the wild type produced. The overproducing phenotype was related to enhancement of the antagonistic activities against several yeasts and pathogenic fungi. Hemolytic activities were also clearly increased in the modified strain. Mass spectrometry analyses of enriched mycosubtilin extracts showed similar patterns of lipopeptides for BBG100 and the wild type. Interestingly, these analyses also revealed a new form of mycosubtilin which was more easily detected in the BBG100 sample. When tested for its biocontrol potential, wild-type strain ATCC 6633 was almost ineffective for reducing a Pythium infection of tomato seedlings. However, treatment of seeds with the BBG100 overproducing strain resulted in a marked increase in the germination rate of seeds. This protective effect afforded by mycosubtilin overproduction was also visualized by the significantly greater fresh weight of emerging seedlings treated with BBG100 compared to controls or seedlings inoculated with the wild-type strain.
http://hdl.handle.net/2268/31330

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Leclère2005.pdfNo commentaryPublisher postprint193.59 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.