Reference : Production and down-stream processing of an extracellular lipase from the yeast Yarro...
Scientific journals : Article
Life sciences : Biotechnology
http://hdl.handle.net/2268/31329
Production and down-stream processing of an extracellular lipase from the yeast Yarrowia lipolytica
English
Fickers, Patrick mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Ongena, MARC mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Destain, Jacqueline mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Weekers, F. [> > > >]
Thonart, Philippe mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
2006
Enzyme & Microbial Technology
Elsevier Science Inc
38
6
756-759
Yes (verified by ORBi)
International
0141-0229
New York
[en] lipase ; bioreactor ; down-stream process ; Yarrowia lipolytica
[en] Lipase constitutes an interesting class of enzyme with many biotechnological applications. However, the development of a fruitful process must be set up to obtained a product compatible with the industrial and commercial needs. Here, we report the development of such a process for the extracellular lipase secreted by the yeast Yarrowia lipolytica. The enzyme production, carried out in a 2000 L bioreactor, led to a lipase activity of approximately 1100 U mL(-1) after 53 h of fermentation. The post-culture treatment, consisting of a centrifugation, a filtration and an ultra-filtration steps, led to 15-fold volume reduction and a 8-fold increased of the lipase activity. Finally, addition of 12% of milk powder and 3% of gum arabic before spray-drying dehydration led to a stable powder with a lipolytic activity of 37,500 U g D.W.(-1). (c) 2005 Elsevier Inc. All rights reserved.
http://hdl.handle.net/2268/31329

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