Reference : Effect of β-cyclodextrin and its derivatives on caveolae disruption, relationships wi...
Scientific journals : Article
Life sciences : Biotechnology
http://hdl.handle.net/2268/31284
Effect of β-cyclodextrin and its derivatives on caveolae disruption, relationships with their cholesterol extraction capacities
English
Castagne, Delphine [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Evrard, Brigitte mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Nusgens, Betty mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire de Biologie des Tissus Conjonctifs > >]
Piel, Géraldine mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
2010
Journal of Inclusion Phenomena and Macrocyclic Chemistry
67
369-376
Yes (verified by ORBi)
International
0923-0750
[en] Cyclodextrins ; endothelial cells ; lipid rafts ; Caveolae ; Cholesterol ; phospholipids
[en] Endothelial cells (HUVEC) were treated with b-cyclodextrin (b-CD) and hydroxypropylated or methylated derivatives solutions to confirm their lack of affinity with phospholipids and their specificity towards cholesterol. Further studies were performed on bovine aortic endothelial cells to assess the effect of b-CDs (mainly methylated derivatives) on membrane microdomains (lipid rafts or caveolae), by detecting the caveolae marker caveolin-1 in fractions of sucrose gradients. A displacement from the lighter to the heavier fractions, characteristic of caveolae disruption, was observed using CDs. The strongest effect was obtained with dimethyl-b-CD, for which an accumulation of caveolin-1 was observed in the bottom of the gradient. Crysmeb and trimethyl-b-CD seemed to have the weaker effects as a significative amount of caveolin-1 was still detected in the light fraction corresponding to caveolae. b-CD and CDs having a degree of methylation a bit lower than 2 showed intermediate effects. The results of the present study on microdomains seem in good correlation with the cell cholesterol extraction capacities of CDs previously determined.
http://hdl.handle.net/2268/31284
10.1007/s10847-009-9718-3

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