[en] The effect of four KATP channel openers (KCOs) on the firing rate of CA1 pyramidal cells and A10 dopaminergic neurons was investigated using extracellular recording techniques in rat brain slices. Pinacidil, lemakalim, diazoxide and a new compound, BPDZ44, had an inhibitory effect on the electrical activity of CA1 pyramidal cells. They all had a similar potency. Only BPDZ44 and diazoxide inhibited the firing rate of A10 dopamine neurons. The sulfonylurea glipizide (1 microM) antagonized the effect of BPDZ44 and diazoxide on A10 neurons but failed to antagonize the effect of KCOs on CA1 pyramidal cells. These results show that differences exist among KCOs in their ability to decrease the electrical activity of various populations of central neurons.
Disciplines :
General & internal medicine Pharmacy, pharmacology & toxicology
Author, co-author :
Scuvée-Moreau, Jacqueline ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Pharmacologie - Département des sciences biomédicales et précliniques
Seutin, Vincent ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Pharmacologie
Vrijens, Bernard ; Université de Liège - ULiège > Département de mathématique > Département de mathématique
Pirotte, Bernard ; Université de Liège - ULiège > Département de pharmacie > Chimie pharmaceutique
De Tullio, Pascal ; Université de Liège - ULiège > Département de pharmacie > Chimie pharmaceutique
Massotte, Laurent ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Albert, Adelin ; Université de Liège - ULiège > Département des sciences de la santé publique > Informatique médicale et biostatistique
Delarge, J
Dresse, Albert ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Effect of potassium channel openers on the firing rate of hippocampal pyramidal cells and A10 dopaminergic neurons in vitro.
AGUILAR-BRYAN, L., NICHOLS, C.G., WECHSLER, S.W., CLEMENT IV, J.P., BOYD III, A.E., GONZALEZ, G., HERRERA-SOSA, H., NGUY, K., BRYAN, J. & NELSON, D.A. (1995) Cloning of the β cell high-affinity sulfonylurea receptor: a regulator of insulin secretion. Science 268, 423-426.
ALZHEIMER, C., SUTOR, B. & TEN BRUGGENCATE, G. (1989) Effects of cromakalim (BRL 34915) on potassium conductances in CA3 neurons of the guinea-pig hippocampus in vitro. Naunyn-Schmiedeberg's Arch. Pharmacol. 340, 465-471.
ASHCROFT, S.J.H. & ASHCROFT, F.M. (1990) Properties and functions of ATP-sensitive K-channels. Cell. Signal. 2, 197-214.
BUNNEY, B.S., SESACK, S.R. & SILVA, N.L. (1987) Midbrain dopaminergic systems: neurophysiology and electrophysiological pharmacology. In: Psychopharmacology: the third generation of progress. MELTZER, H.Y. Ed. Raven Press. New York, pp. 113-126.
CREPEL, V., ROVIRA, C. & BEN-ARI, Y. (1993) The K+ channel opener diazoxide enhances glutamatergic currents and reduces GABAergic currents in hippocampal neurons. J. Neurophys. 69, 494-503.
DIGGLE, P.J., LIANG, K.Y. & ZEGER, Q.L. (1994) in: Analysis of longitudinal data. ATKINSON, A.C. et al. Eds. Clarendon Press. Oxford Science Publication.
DUNNE, M.J., YULE, D.I., GALLACHER, D.V. & PETERSON, O.H. (1990) Comparative study of the effects of cromakalim (BRL 34915) and diazoxide on membrane potential, [Ca++]i and ATP-sensitive potassium currents in insulin-secreting cells. J. Membr. Biol. 114, 53-60.
EDWARDS, G. & WESTON, A.M. (1993) The pharmacology of ATP-sensitive potassium channels. Annu. Rev. Pharmacol. Toxicol. 33, 597-637.
ERDEMLI, G. & KRNJEVIC, K. (1993) Diazoxide suppresses slowly-inactivating outward and inward currents in CA1 hippocampal neurones. Neuro Report 5, 249-251.
ERDEMLI, G. & KRNJEVIC, K. (1994) Actions of cromakalim on outward currents of CA1 neurones in hippocampal slices. Br. J. Pharmacol. 113, 411-418.
GEHLERT, D.R. & ROBERTSON, D.W. (1994) ATP sensitive potassium channels: potential drug targets in neuropsychopharmacology. Prog. Neuro-Psychopharmacol. & Biol. Psychiat. 18, 1093-1102.
HAAS, H.L., SCHAERER, B. & VOSMANSKY, M. (1979) A simple perfusion chamber for the study of nervous tissue slices in vitro. J Neurosci. Methods 1, 323-325.
HAMILTON, T.C. & WESTON, A.H. (1989) Cromakalim, nicorandil and pinacidil: novel drugs which open potassium channels in smooth muscle. Gen. Pharmacol. 20, 1-9.
KANDEL, E.R. & SPENCER, W.A. (1961) Electrophysiology of hippocampal neurons II. After-potentials and repetitive firing. J. Neurophysiol. 24, 243-259.
LAZDUNSKI, M. (1994) ATP-sensitive potassium channels: an overview. J. Cardiovasc. Pharmacol. 24, suppl. 4, S1-S5.
NEWGREEN, D.T., BRAY, K.M., MCHARG, A.D., WESTON, A.H., DUTY, S., BROWN, B.S., KAY, P.B., EDWARDS, G., LONGMORE, J. & SOUTHERTON, J.S. (1990) The action of diazoxide and minoxidil sulphate on rat blood vessels: a comparison with cromakalim. Br. J. Pharmacol. 100, 605-613.
PIROTTE, B., DE TULLIO, P., LEBRUN, P., ANTOINE, M.H., FONTAINE, J., MASEREEL, B., SCHYNTS, M., DUPONT, L., HERCHUELZ, A. & DELARGE, J. (1993) 3-(Alkylamino)-4H-pyrido[4,3-e]-1,2,4-thiadiazine 1,1-dioxides as powerful inhibitors of insulin release from rat pancreatic B-cells: a new class of potassium channel openers? J. Med. Chem. 36, 3211-3213.
PIROTTE, B., FONTAINE, J. & LEBRUN, P. (1995) Recent advances in the chemistry of potassium channel openers. Current Med. Chem. 2, 573-582.
QUAST, U. (1993) Do the K+ channel openers relax smooth muscle by opening K+ channels? Trends Pharmacol. Sci. 14, 332-337.
SEUTIN, V., SCUVEE-MOREAU, J., GIESBERS, I., MASSOTTE, L. & DRESSE, A. (1990a) Effect of BHT 920 on monoaminergic neurons of the rat brain: an electrophysiological in vivo and in vitro study. Naunyn-Schmiedeberg's Arch. Pharmacol. 342, 502-507.
SEUTIN, V., VERBANCK, P., MASSOTTE, L. & DRESSE, A. (1990b) Evidence for the presence of N-methyl-D-aspartate receptors in the ventral tegmental area of the rat: an electrophysiological in vitro study. Brain Res. 514, 147-150.
SEUTIN, V., SHEN, K.Z., NORTH, R.A. & JOHNSON, S.W. (1996) Sulfonylurea-sensitive potassium current evoked by sodium-loading in rat midbrain dopamine neurons. Neuroscience 7, 709-719.
STURGESS, N.C., ASHFORD, M.L.J., COOK, D.L. & HALES, C.N. (1985) The sulfonylurea receptor may be an ATP-sensitive potassium channel. Lancet 8453, 474-475.
TRUBE, G., RORSMAN, P. & OHNO-SHOSAKU, T. (1986) Opposite effects of tolbutamide and diazoxide on the ATP-dependent K+ channel in mouse pancreatic β-cells. Pflügers Arch. 407, 493-499.
WATTS, A.E., HICKS, G.A. & HENDERSON, G. (1995) Putative pre-and postsynaptic ATP-sensitive potassium channels in the rat substantia nigra in vitro. J. Neurosci. 15, 3065-3074.
ZINI, S., TREMBLAY, E., POLLARD, H., MOREAU, J. & BEN-ARI, Y. (1993) Regional distribution of sulfonylurea receptors in the brain of rodent and primate. Neuroscience 55, 1085-1091.