Reference : Validation and interpretation of CALUX as a tool for the estimation of dioxin-like ac...
Scientific journals : Article
Life sciences : Aquatic sciences & oceanology
Life sciences : Environmental sciences & ecology
http://hdl.handle.net/2268/31147
Validation and interpretation of CALUX as a tool for the estimation of dioxin-like activity in marine biological matrixes.
English
Windal, Isabelle mailto [Institut Scientifique de la Santé publique = Wetenschappelijk Instituut Volksgezondheid (Belgique) - ISP = WIV > > > > Denrées alimentaires > >]
Van Wouwe, Nathalie [Institut Scientifique de la Santé publique = Wetenschappelijk Instituut Volksgezondheid (Belgique) - ISP = WIV > > > > > >]
Eppe, Gauthier mailto [Université de Liège - ULg > > Centre interfac. d'analyse des résidus en traces (CART) >]
Xhrouet, Céline mailto [Université de Liège - ULg > Département de chimie (sciences) > Département de chimie (sciences) >]
Debacker, Virginie mailto [Université de Liège - ULg > Département des sciences et gestion de l'environnement > Ecologie animale et écotoxicologie >]
Baeyens, Willy [> > > >]
De Pauw, Edwin mailto [Université de Liège - ULg > > Chimie physique, spectrométrie de masse >Centre interfac. d'analyse des résidus en traces (CART) > > >]
Goeyens, Léo mailto [Vrije Universiteit Brussel - VUB > > > > > >]
15-Mar-2005
Environmental Science & Technology
Amer Chemical Soc
39
6
1741-1748
Yes (verified by ORBi)
International
0013-936X
Washington
[en] CALUX ; PCDD/Fs ; GC-HRMS ; Fish ; Mussel ; starfish ; seabird ; marine mammal
[en] Among the different analytical tools proposed as an alternative to the very expensive gas chromatography high-resolution mass spectrometry (GC-HRMS) analyses of polychlorodibenzo-p-dioxin and polychlorodibenzofurans, Chemically Activated LUciferase gene eXpression (CALUX) in vitro cell bioassay is very promising. It allows the analyses of a high number of samples since it is relatively fast, inexpensive, and sensitive. However, this technique is not yet widely applied for screening or environmental monitoring. The main reasons are probably the lack of validation and the difficulty in interpreting the global biological response of the bioassay. In this paper, the strict quality control criteria set up for the validation of CALUX are described. The validation has shown good repeatability (relative standard deviation (RSD) = 9%) and good within-lab reproducibility (RSD = 15%) of the results. The quantification limit, in the conditions applied in this paper, is 1.25 pg CALUX-TEQ/g fat. Comparison of CALUX and GC-HRMS analysis was made for various marine matrixes (fishes, mussels, starfishes, sea birds, and marine mammals). Good correlations are usually observed, but there are systematic differences between the results. Attempts are made to identify the origin of the discrepancy between the two methods.
'Impulse Program in Marine Sciences supported by the Belgian State, the Prime's Minister Services, scientific, Technical and Cultural Affairs (MN/12/95).'
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/31147

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Environ. Sci. Technol. 2005.pdfPublisher postprint203.61 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.