Article (Scientific journals)
The beneficial role of different auxins and polyamines at successive stages of somatic embryo formation and development of Panax ginseng in vitro
Kevers, Claire; Gaspar, Thomas; Dommes, Jacques
2002In Plant Cell, Tissue and Organ Culture, 70 (2), p. 181-188
Peer reviewed
 

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Keywords :
3-(benzo[b]selenyl)acetic acid; somatic embryogenesis; spermidine
Abstract :
[en] The production of viable plantlets via somatic embryogenesis in Panax ginseng requires different culture media corresponding to successive developmental stages. The effects of several auxins and polyamines have been tested at various steps. Multiplication of the embryogenic root-derived callus has been optimized on half-strength MS medium supplemented with 3-(benzo[b]selenyl)acetic acid (BSAA, a synthetic auxin) and kinetin; exogenously applied polyamines were deleterious at this stage, causing browning of the callus, diminished capacity of embryo initiation, and an increased tendency to hyperhydricity. BSAA again appeared to be the most favourable auxin at the initiation stage, but here its action was reinforced by the presence of polyamines, spermidine being the most efficient. Among the auxin needed at the next step, i.e., for the regeneration of embryos, the two seleniated auxins BSAA and seleniated 2,4-dichlorophenoxyacetic acid (2,4-D-Se) were the more efficient. For the harmonious development of plantlets, i.e., the simultaneous outgrowth of shoots and roots, the polyamines were favourable, with a greater efficiency for spermine.
Disciplines :
Phytobiology (plant sciences, forestry, mycology...)
Microbiology
Biotechnology
Author, co-author :
Kevers, Claire ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Gaspar, Thomas ;  Botanique, radiobotanique & serres expér.
Dommes, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Language :
English
Title :
The beneficial role of different auxins and polyamines at successive stages of somatic embryo formation and development of Panax ginseng in vitro
Publication date :
August 2002
Journal title :
Plant Cell, Tissue and Organ Culture
ISSN :
0167-6857
eISSN :
1573-5044
Publisher :
Kluwer Academic Publ, Dordrecht, Netherlands
Volume :
70
Issue :
2
Pages :
181-188
Peer reviewed :
Peer reviewed
Available on ORBi :
since 28 December 2008

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