Article (Scientific journals)
Human bone marrow adipocytes block granulopoiesis through neuropilin-1-induced granulocyte colony-stimulating factor inhibition.
Belaid-Choucair, Zakia; Lepelletier, Yves; Poncin, Géraldine et al.
2008In Stem Cells, 26 (6), p. 1556-64
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Keywords :
Adipocytes/cytology/drug effects/physiology; Animals; Antigens, CD/physiology; Antigens, CD34/physiology; Bone Marrow Cells/cytology/drug effects/physiology; Cell Differentiation; DNA Primers; Gene Expression Regulation; Granulocyte Colony-Stimulating Factor/antagonists & inhibitors/genetics; Humans; Intercellular Signaling Peptides and Proteins/genetics; Macrophages/cytology/physiology; Membrane Proteins/genetics; Mice; Mice, Inbred NOD; Mice, SCID; Neuropilin-1/physiology; Reverse Transcriptase Polymerase Chain Reaction
Abstract :
[en] Adipocytes are part of hematopoietic microenvironment, even though up to now in humans, their role in hematopoiesis is still questioned. We have previously shown that accumulation of fat cells in femoral bone marrow (BM) coincides with increased expression of neuropilin-1 (NP-1), while it is weakly expressed in hematopoietic iliac crest BM. Starting from this observation, we postulated that adipocytes might exert a negative effect on hematopoiesis mediated through NP-1. To test this hypothesis, we set up BM adipocytes differentiated into fibroblast-like fat cells (FLFC), which share the major characteristics of primitive unilocular fat cells, as an experimental model. As expected, FLFCs constitutively produced macrophage colony stimulating factor and induced CD34(+) differentiation into macrophages independently of cell-to-cell contact. By contrast, granulopoiesis was hampered by cell-to-cell contact but could be restored in transwell culture conditions, together with granulocyte colony stimulating factor production. Both functions were also recovered when FLFCs cultured in contact with CD34(+) cells were treated with an antibody neutralizing NP-1, which proved its critical implication in contact inhibition. An inflammatory cytokine such as interleukin-1 beta or dexamethasone modulates FLFC properties to restore granulopoiesis. Our data provide the first evidence that primary adipocytes exert regulatory functions during hematopoiesis that might be implicated in some pathological processes. Disclosure of potential conflicts of interest is found at the end of this article.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Belaid-Choucair, Zakia ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
Lepelletier, Yves
Poncin, Géraldine ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
Thiry, Albert ;  Centre Hospitalier Universitaire de Liège - CHU > Anatomie pathologique
Humblet, Chantal ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
Maachi, M.
Beaulieu, Aurore ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
Schneider, Elke
Briquet, Alexandra ;  Université de Liège - ULiège > Département des sciences cliniques > Hématologie
Mineur, Pierre ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal.
Lambert, Charles ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal.
Mendes-Da-Cruz, Daniella
Ahui, Marie Louise
Asnafi, Vahid
Dy, Michel
Boniver, Jacques ;  Centre Hospitalier Universitaire de Liège - CHU > Anatomie pathologique
Nusgens, Betty ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Hermine, Olivier
Defresne, Marie-Paule ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
More authors (9 more) Less
Language :
English
Title :
Human bone marrow adipocytes block granulopoiesis through neuropilin-1-induced granulocyte colony-stimulating factor inhibition.
Publication date :
2008
Journal title :
Stem Cells
ISSN :
1066-5099
eISSN :
1549-4918
Publisher :
AlphaMed Company, Inc., Miamisburg, United States - Ohio
Volume :
26
Issue :
6
Pages :
1556-64
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 February 2010

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