Reference : Human bone marrow adipocytes block granulopoiesis through neuropilin-1-induced granulocy...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/30809
Human bone marrow adipocytes block granulopoiesis through neuropilin-1-induced granulocyte colony-stimulating factor inhibition.
English
Belaid-Choucair, Zakia [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
Lepelletier, Yves [> > > >]
Poncin, Géraldine mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
Thiry, Albert mailto [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
Humblet, Chantal mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
Maachi, M. [ > > ]
Beaulieu, Aurore mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
Schneider, Elke [> > > >]
Briquet, Alexandra mailto [Université de Liège - ULg > Département des sciences cliniques > Hématologie >]
Mineur, Pierre mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal. >]
Lambert, Charles mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal. >]
Mendes-Da-Cruz, Daniella [> > > >]
Ahui, Marie Louise [> > > >]
Asnafi, Vahid [> > > >]
Dy, Michel [> > > >]
Boniver, Jacques mailto [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
Nusgens, Betty mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Hermine, Olivier [> > > >]
Defresne, Marie-Paule mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
2008
Stem Cells
AlphaMed Company, Inc.
26
6
1556-64
Yes (verified by ORBi)
International
1066-5099
1549-4918
Miamisburg
OH
[en] Adipocytes/cytology/drug effects/physiology ; Animals ; Antigens, CD/physiology ; Antigens, CD34/physiology ; Bone Marrow Cells/cytology/drug effects/physiology ; Cell Differentiation ; DNA Primers ; Gene Expression Regulation ; Granulocyte Colony-Stimulating Factor/antagonists & inhibitors/genetics ; Humans ; Intercellular Signaling Peptides and Proteins/genetics ; Macrophages/cytology/physiology ; Membrane Proteins/genetics ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Neuropilin-1/physiology ; Reverse Transcriptase Polymerase Chain Reaction
[en] Adipocytes are part of hematopoietic microenvironment, even though up to now in humans, their role in hematopoiesis is still questioned. We have previously shown that accumulation of fat cells in femoral bone marrow (BM) coincides with increased expression of neuropilin-1 (NP-1), while it is weakly expressed in hematopoietic iliac crest BM. Starting from this observation, we postulated that adipocytes might exert a negative effect on hematopoiesis mediated through NP-1. To test this hypothesis, we set up BM adipocytes differentiated into fibroblast-like fat cells (FLFC), which share the major characteristics of primitive unilocular fat cells, as an experimental model. As expected, FLFCs constitutively produced macrophage colony stimulating factor and induced CD34(+) differentiation into macrophages independently of cell-to-cell contact. By contrast, granulopoiesis was hampered by cell-to-cell contact but could be restored in transwell culture conditions, together with granulocyte colony stimulating factor production. Both functions were also recovered when FLFCs cultured in contact with CD34(+) cells were treated with an antibody neutralizing NP-1, which proved its critical implication in contact inhibition. An inflammatory cytokine such as interleukin-1 beta or dexamethasone modulates FLFC properties to restore granulopoiesis. Our data provide the first evidence that primary adipocytes exert regulatory functions during hematopoiesis that might be implicated in some pathological processes. Disclosure of potential conflicts of interest is found at the end of this article.
http://hdl.handle.net/2268/30809
10.1634/stemcells.2008-0068
http://stemcells.alphamedpress.org/cgi/content/full/26/6/1556

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