ORBi: Jacobs Nathalie - Generation of T lymphocytes from the epithelium and stroma of squamous pre-neoplastic lesions of the uterine cervix.

Reference : Generation of T lymphocytes from the epithelium and stroma of squamous pre-neoplastic le...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biotechnology Human health sciences : Immunology & infectious disease
	To cite this reference:	http://hdl.handle.net/2268/30783

Title :	Generation of T lymphocytes from the epithelium and stroma of squamous pre-neoplastic lesions of the uterine cervix.
Language :	English
Author, co-author :	Jacobs, Nathalie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Giannini, Sandra [Université de Liège - ULg > Département des sciences cliniques > Département des sciences cliniques >]
	Al-Saleh, Walid [> > > >]
	Hubert, Pascale [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Boniver, Jacques [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
	Delvenne, Philippe [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Publication date :	1999
Journal title :	Journal of Immunological Methods
Publisher :	Elsevier Science
Volume :	223
Issue/season :	1
Pages :	123-9
Audience :	International
ISSN :	0022-1759
Keywords :	[en] Carcinoma in Situ/pathology ; Carcinoma, Squamous Cell/immunology/pathology ; Cell Separation ; Cells, Cultured ; Cervical Intraepithelial Neoplasia/immunology ; Epithelial Cells/immunology ; Female ; Humans ; Immunohistochemistry ; Immunophenotyping ; Interleukin-2/pharmacology ; Lymphocyte Activation ; Precancerous Conditions/immunology ; Stromal Cells/immunology/pathology ; T-Lymphocyte Subsets/immunology/pathology ; Uterine Cervical Neoplasms/immunology
Abstract :	[en] In this study, we have developed a simple and efficient technique for the isolation of viable lymphocytes from the epithelium and stroma of small pre-neoplastic squamous intraepithelial lesions (SIL) of the uterine cervix. Following the separation of the epithelium from the stroma using dispase II, both biopsy fragments were used to generate T lymphocytes. The stroma-derived lymphocytes were obtained by collecting and culturing the cells migrating out of the biopsy in the presence of IL2 (50 U/ml). An average of 0.7 x 10(6) and 1.4 x 10(6) lymphocytes could be obtained after 20 and 30 days of culture, respectively. For the expansion of lymphocytes derived from the pre-neoplastic epithelium (SIL) it was necessary to use a combination of irradiated peripheral blood mononuclear cells (PBMC) as a feeder layer with PHA (0.1%), in addition to IL2 (50 U/ml). Interestingly, these lymphocytes could be obtained using either allogeneic or syngeneic PBMCs. With this protocol, we were able to generate up to 100 x 10(6) lymphocytes from the epithelium, the majority of which were T lymphocytes.
Funders :	Centre de Recherche Interuniversitaire en Vaccinologie with a grant from the Walloon Region and SmithKline Beecham Bio- logicals ; Belgian Fund for Medical Scientific Research ; B104-CT98-0097 EU contract ; Oeuvre Belge du Cancer ; Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Target :	Researchers ; Students
Permalink :	http://hdl.handle.net/2268/30783

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