Reference : Hyperhydricity of Prunus avium shoots cultured on gelrite: a controlled stress response
Scientific journals : Article
Life sciences : Biotechnology
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/2268/3078
Hyperhydricity of Prunus avium shoots cultured on gelrite: a controlled stress response
English
Franck, Thierry mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Kevers, Claire mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
Gaspar, Thomas mailto [> > Botanique, radiobotanique & serres expér. >]
Dommes, Jacques mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
Deby, Carol [ > > ]
Greimers, Roland mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Serteyn, Didier mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Deby-Dupont, Ginette [Université de Liège > Sciences > Centre de l'Oxygène > >]
Jun-2004
Plant Physiology & Biochemistry
Editions Scientifiques Medicales Elsevier
42
6
519-527
Yes (verified by ORBi)
International
0981-9428
Paris
[en] autophagy ; cell integrity ; free fatty acids ; glutathione peroxidase ; hyperhydricity Prunus avium ; stress markers
[en] Hyperhydricity is a physiological disorder frequently affecting shoots vegetatively propagated in vitro. Hyperhydric shoots are characterised by a translucent aspect due to a chlorophyll deficiency, a not very developed cell wall and a high water content. Hyperhydricity of Prunus avium shoots was expressed in vitro in one multiplication cycle by replacing the gelling agent agar (normal shoots: NS) by gelrite (hyperhydric shoots: HS). P. avium shoots evolving towards the hyperhydric state produced higher amounts of ethylene, polyamines (PAs) and proline, which are substances considered as stress markers. A higher activity of glutathione peroxidase (GPX; EC 1.11.1.9), involved in organic hydroperoxide elimination, suggested an increased production of these compounds in HS. The unchanged free fatty acid composition indicated no HS membrane damages compared to NS. The ploidy level of HS nuclei was not affected, but the bigger size and the lower percentage of nuclei during the S phase suggested a slowing down of the cell cycle. The results argued for a stress response of the HS, but no signs of oxidative damages of lipid membrane and nucleus were observed. The discussion points out paradoxical results in a classical analysis of stress and suggests an alternative way of defense mechanisms in HS, involving homeostatic regulation and controlled degradation processes to maintain integrity and vital functions of the cell. (C) 2004 Elsevier SAS. All rights reserved.
Researchers ; Professionals
http://hdl.handle.net/2268/3078
10.1016/j.plaphy.2004.05.003

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