Reference : Mutants of Chlamydomonas reinhardtii deficient in mitochondrial complex I: Characteri...
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/30424
Mutants of Chlamydomonas reinhardtii deficient in mitochondrial complex I: Characterization of two mutations affecting the nd1 coding sequence
English
Remacle, Claire mailto [Université de Liège - ULg > Département des sciences de la vie > Génétique >]
Baurain, Denis mailto [Université de Liège - ULg > Département de Botanique > Génétique des microorganismes > >]
Cardol, Pierre mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie végétale >]
Matagne, René-Fernand mailto [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]
Jul-2001
Genetics
158
3
1051-60
Yes (verified by ORBi)
International
0016-6731
[en] The mitochondrial rotenone-sensitive NADH:ubiquinone oxidoreductase (complex I) comprises more than 30 subunits, the majority of which are encoded by the nucleus. In Chlamydomonas reinhardtii, only five components of complex I are coded for by mitochondrial genes. Three mutants deprived of complex I activity and displaying slow growth in the dark were isolated after mutagenic treatment with acriflavine. A genetical analysis demonstrated that two mutations (dum20 and dum25) affect the mitochondrial genome whereas the third mutation (dn26) is of nuclear origin. Recombinational analyses showed that dum20 and dum25 are closely linked on the genetic map of the mitochondrial genome and could affect the nd1 gene. A sequencing analysis confirmed this conclusion: dum20 is a deletion of one T at codon 243 of nd1; dum25 corresponds to a 6-bp deletion that eliminates two amino acids located in a very conserved hydrophilic segment of the protein.
http://hdl.handle.net/2268/30424

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