Peptide-Induced Fractal Assembly of Silver Nanoparticles for Visual Detection of Disease Biomarkers.

coalescence; colorimetric sensing; fractal structures; peptides; sensor-array silver nanomaterials; Silver; Peptides; Peptide Hydrolases; Biomarkers; Humans; Silver/chemistry; Colorimetry; Limit of Detection; Fractals; SARS-CoV-2; Metal Nanoparticles/chemistry; COVID-19/diagnosis; Amino-acids; Color changes; Diffusion limited aggregation; Disease biomarker; Sensor-array silver nanomaterial; Sensors array; Visual detection; COVID-19; Metal Nanoparticles; Materials Science (all); Engineering (all); Physics and Astronomy (all); General Physics and Astronomy; General Engineering; General Materials Science

Abstract :

[en] We report the peptide-programmed fractal assembly of silver nanoparticles (AgNPs) in a diffusion-limited aggregation (DLA) mode, and this change in morphology generates a significant color change. We show that peptides with specific repetitions of defined amino acids (i.e., arginine, histidine, or phenylalanine) can induce assembly and coalescence of the AgNPs (20 nm) into a hyperbranched structure (AgFSs) (∼2 μm). The dynamic process of this assembly was systematically investigated, and the extinction of the nanostructures can be modulated from 400 to 600 nm by varying the peptide sequences and molar ratio. According to this rationale, two strategies of SARS-CoV-2 detection were investigated. The activity of the main protease (Mpro) involved in SARS-CoV-2 was validated with a peptide substrate that can bridge the AgNPs after the proteolytic cleavage. A sub-nanomolar limit of detection (0.5 nM) and the capacity to distinguish by the naked eye in a wide concentration range (1.25-30 nM) were achieved. Next, a multichannel sensor-array based on multiplex peptides that can visually distinguish SARS-CoV-2 proteases from influenza proteases in doped human samples was investigated.

Disciplines :

Chemistry

Author, co-author :

Retout, Maurice ; Department of NanoEngineering, Materials Science and Engineering Program, University of California, San Diego, United States

Mantri, Yash ; Department of Bioengineering, University of California, San Diego, United States

Jin, Zhicheng ; Department of NanoEngineering, Materials Science and Engineering Program, University of California, San Diego, United States

Zhou, Jiajing ; Department of NanoEngineering, Materials Science and Engineering Program, University of California, San Diego, United States

Noël, Grégoire ; Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs

Donovan, Brian; Department of NanoEngineering, Materials Science and Engineering Program, University of California, San Diego, United States

Yim, Wonjun ; Department of NanoEngineering, Materials Science and Engineering Program, University of California, San Diego, United States

Jokerst, Jesse V ; Department of NanoEngineering, Materials Science and Engineering Program, University of California, San Diego, United States

Language :

English

Title :

Peptide-Induced Fractal Assembly of Silver Nanoparticles for Visual Detection of Disease Biomarkers.

Publication date :

26 April 2022

Journal title :

ACS Nano

ISSN :

1936-0851

eISSN :

1936-086X

Publisher :

American Chemical Society, United States

Volume :

Issue :

Pages :

6165 - 6175

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://pubs.acs.org/doi/pdf/10.1021/acsnano.1c11643

Funders :

NIH - National Institutes of Health [US-MD] [US-MD]

Funding text :

J. Jokerst acknowledges funding from NIH under grants R01DE031114, R21AG0657776-01S1, R21AI157957, and DP2HL137187-S1. We also acknowledge infrastructure support under NIH S10 OD023555. M.R. acknowledges the Wallonie-Bruxelles International (WBI) of the Fédération Wallonie-Bruxelles for financial support. Figures 1, 4, and 5 were made using BioRender.com.

Available on ORBi :

since 15 June 2023

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