Reference : CYTODUCTION IN CHLAMYDOMONAS-REINHARDTII
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/30393
CYTODUCTION IN CHLAMYDOMONAS-REINHARDTII
English
Matagne, René-Fernand mailto [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]
Remacle, Claire mailto [Université de Liège - ULg > Département des sciences de la vie > Génétique >]
DINANT, M. [> > > >]
1991
Proceedings of the National Academy of Sciences of the United States of America
National Academy of Sciences
88
16
7447-7450
Yes (verified by ORBi)
International
0027-8424
1091-6490
Washington
DC
[en] After conjugation between Chlamydomonas gametes of opposite mating type, a transient dikaryon is formed. The two nuclei fuse within 4-6 hr after mating. The young diploid zygote differentiates into dormant zygospore competent to complete meiosis, or more rarely (2-10% of cases) it undergoes mitosis to produce a stable diploid progeny. We here bring genetical, biochemical, and cytological evidence that among the mitotic zygotes, a large proportion of them undergo cytokinesis without fusion of the nuclei - a process that has been termed "cytoduction." By using appropriate genetic markers, haploid cytoductants that possess the nuclear genotype of one parent and the chloroplast marker of the other parent can easily be isolated. Genetical analysis and hybridization experiments moreover show that many haploid cytoductants transmit the chloroplast DNA molecules of both parents and that, as in diploids, these DNA copies occasionally recombine. This process of cytoduction extends the life cycle of Chlamydomonas and provides new tools for its genetic analysis.
http://hdl.handle.net/2268/30393
also: http://hdl.handle.net/2268/35968

There is no file associated with this reference.

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.