|Reference : CYTODUCTION IN CHLAMYDOMONAS-REINHARDTII|
|Scientific journals : Article|
|Life sciences : Genetics & genetic processes|
|CYTODUCTION IN CHLAMYDOMONAS-REINHARDTII|
|Matagne, René-Fernand [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]|
|Remacle, Claire [Université de Liège - ULg > Département des sciences de la vie > Génétique >]|
|DINANT, M. [> > > >]|
|Proceedings of the National Academy of Sciences of the United States of America|
|National Academy of Sciences|
|Yes (verified by ORBi)|
|[en] After conjugation between Chlamydomonas gametes of opposite mating type, a transient dikaryon is formed. The two nuclei fuse within 4-6 hr after mating. The young diploid zygote differentiates into dormant zygospore competent to complete meiosis, or more rarely (2-10% of cases) it undergoes mitosis to produce a stable diploid progeny. We here bring genetical, biochemical, and cytological evidence that among the mitotic zygotes, a large proportion of them undergo cytokinesis without fusion of the nuclei - a process that has been termed "cytoduction." By using appropriate genetic markers, haploid cytoductants that possess the nuclear genotype of one parent and the chloroplast marker of the other parent can easily be isolated. Genetical analysis and hybridization experiments moreover show that many haploid cytoductants transmit the chloroplast DNA molecules of both parents and that, as in diploids, these DNA copies occasionally recombine. This process of cytoduction extends the life cycle of Chlamydomonas and provides new tools for its genetic analysis.|
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