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Article (Scientific journals)
A promoter element active in run-off transcription controls the expression of two cistrons of nad and rps genes in Nicotiana sylvestris mitochondria
Lelandais, C.; Gutierres, S.; Mathieu, C. et al.
1996In Nucleic Acids Research, 24 (23), p. 4798-4804
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Abstract :
[en] The expression of two mitochondrial gene clusters (orf87-nad3-nad1/A and orf87-nad3-rps 12) was studied in Nicotiana sylvestris. 5' and 3' termini of transcripts were mapped by primer extension and nuclease S1 protection. Processing and transcription initiation sites were differentiated by in vitro phosphorylation and capping experiments. A transcription initiation site, present in both gene clusters, was found 213 nucleotides upstream of orf87. This promoter element matches the consensus motif for dicotyledonous mitochondrial promoters and initiates run-off transcription in a pea mitochondrial purified protein fraction, Processing sites were identified 5' of nad3, nad1/A and rps12 respectively. These results suggest that (i) the expression of the two cistrons is only controlled by one duplicated promoter element, and (ii) multiple processing events are required to produce monocistronic nad3, nad1/A and rps12 transcripts.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Lelandais, C.
Gutierres, S.
Mathieu, C.
Vedel, F.
Remacle, Claire  ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique
MarechalDrouard, L.
Brennicke, A.
Binder, S.
Chetrit, P.
Language :
English
Title :
A promoter element active in run-off transcription controls the expression of two cistrons of nad and rps genes in Nicotiana sylvestris mitochondria
Publication date :
1996
Journal title :
Nucleic Acids Research
ISSN :
0305-1048
eISSN :
1362-4962
Publisher :
Oxford University Press, Oxford, United Kingdom
Volume :
24
Issue :
23
Pages :
4798-4804
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 November 2009

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