Reference : A promoter element active in run-off transcription controls the expression of two cistro...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/30388
A promoter element active in run-off transcription controls the expression of two cistrons of nad and rps genes in Nicotiana sylvestris mitochondria
English
Lelandais, C. [> > > >]
Gutierres, S. [> > > >]
Mathieu, C. [> > > >]
Vedel, F. [> > > >]
Remacle, Claire mailto [Université de Liège - ULg > Département des sciences de la vie > Génétique >]
MarechalDrouard, L. [> > > >]
Brennicke, A. [> > > >]
Binder, S. [> > > >]
Chetrit, P. [> > > >]
1996
Nucleic Acids Research
Oxford University Press
24
23
4798-4804
Yes (verified by ORBi)
International
0305-1048
1362-4962
Oxford
United Kingdom
[en] The expression of two mitochondrial gene clusters (orf87-nad3-nad1/A and orf87-nad3-rps 12) was studied in Nicotiana sylvestris. 5' and 3' termini of transcripts were mapped by primer extension and nuclease S1 protection. Processing and transcription initiation sites were differentiated by in vitro phosphorylation and capping experiments. A transcription initiation site, present in both gene clusters, was found 213 nucleotides upstream of orf87. This promoter element matches the consensus motif for dicotyledonous mitochondrial promoters and initiates run-off transcription in a pea mitochondrial purified protein fraction, Processing sites were identified 5' of nad3, nad1/A and rps12 respectively. These results suggest that (i) the expression of the two cistrons is only controlled by one duplicated promoter element, and (ii) multiple processing events are required to produce monocistronic nad3, nad1/A and rps12 transcripts.
http://hdl.handle.net/2268/30388

There is no file associated with this reference.

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.