Reference : Characterization of pentraxin 3 in the horse and its expression in airways.
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/30232
Characterization of pentraxin 3 in the horse and its expression in airways.
English
Ramery, Eve mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie - Biochimie >]
Fievez, Laurence mailto [Université de Liège - ULg > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire >]
Fraipont, Audrey mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie >]
Bureau, Fabrice mailto [Université de Liège - ULg > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire >]
Lekeux, Pierre mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie - Doyen de la Faculté de Médecine vétérinaire >]
2010
Veterinary Research
EDP Sciences
41
2
18
Yes (verified by ORBi)
International
0928-4249
Les Ulis
France
[en] PTX3 ; horse ; airway ; inflammation
[en] The long pentraxin 3 (PTX3) plays an important role in host defence and its over-expression may contribute to airway injury. The aim of the present study was therefore to characterize in more detail PTX3 and its expression in the horses airway. Six healthy horses and six horses affected by recurrent airway obstruction (R.A.O.) were submitted to a dusty environment challenge. PTX3 DNA and cDNA were cloned and sequenced. PTX3 expression was evaluated by RT-qPCR, western blotting and immuno-histochemistry in bronchoalveolar lavage fluid (BALF) cells, BALF supernatant and bronchial epithelial cells. An alternative splicing of the second exon of PTX3 occurred, resulting in two forms of the protein: spliced (32 kDa) and full length (42 kDa). PTX3 was detected in BALF macrophages, neutrophils and bronchial epithelial cells. It was over-expressed in the BALF supernatant from R.A.O.-affected horses in crisis. However, dust was unable to induce PTX3 in BALF cells ex vivo, indicating that dust is an indirect inducer of PTX3. Dust exposure in-vivo induced PTX3 in BALF macrophages but there was no significant difference between healthy and R.A.O.-affected horses. Conversely, PTX3 was over-expressed in the bronchial epithelial cells from R.A.O-affected horses in crisis. These data indicate a differential regulatory mechanism in inflammatory and bronchial epithelial cells and offer therapeutically interesting perspectives.
Researchers ; Professionals
http://hdl.handle.net/2268/30232
10.1051/vetres/2009066

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
RameryE_VetRes_2010.pdfPublisher postprint975.02 kBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.