Article (Scientific journals)
Characterization of pentraxin 3 in the horse and its expression in airways.
Ramery, Eve; Fievez, Laurence; Fraipont, Audrey et al.
2010In Veterinary Research, 41 (2), p. 18
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Keywords :
PTX3; horse; airway; inflammation
Abstract :
[en] The long pentraxin 3 (PTX3) plays an important role in host defence and its over-expression may contribute to airway injury. The aim of the present study was therefore to characterize in more detail PTX3 and its expression in the horses airway. Six healthy horses and six horses affected by recurrent airway obstruction (R.A.O.) were submitted to a dusty environment challenge. PTX3 DNA and cDNA were cloned and sequenced. PTX3 expression was evaluated by RT-qPCR, western blotting and immuno-histochemistry in bronchoalveolar lavage fluid (BALF) cells, BALF supernatant and bronchial epithelial cells. An alternative splicing of the second exon of PTX3 occurred, resulting in two forms of the protein: spliced (32 kDa) and full length (42 kDa). PTX3 was detected in BALF macrophages, neutrophils and bronchial epithelial cells. It was over-expressed in the BALF supernatant from R.A.O.-affected horses in crisis. However, dust was unable to induce PTX3 in BALF cells ex vivo, indicating that dust is an indirect inducer of PTX3. Dust exposure in-vivo induced PTX3 in BALF macrophages but there was no significant difference between healthy and R.A.O.-affected horses. Conversely, PTX3 was over-expressed in the bronchial epithelial cells from R.A.O-affected horses in crisis. These data indicate a differential regulatory mechanism in inflammatory and bronchial epithelial cells and offer therapeutically interesting perspectives.
Disciplines :
Veterinary medicine & animal health
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Ramery, Eve ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie - Biochimie
Fievez, Laurence ;  Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Fraipont, Audrey ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie
Bureau, Fabrice ;  Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Lekeux, Pierre ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie - Doyen de la Faculté de Médecine vétérinaire
Language :
English
Title :
Characterization of pentraxin 3 in the horse and its expression in airways.
Publication date :
2010
Journal title :
Veterinary Research
ISSN :
0928-4249
eISSN :
1297-9716
Publisher :
EDP Sciences, Les Ulis, France
Volume :
41
Issue :
2
Pages :
18
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 November 2009

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