Article (Scientific journals)
Localization of Nopp140 within mammalian cells during interphase and mitosis.
Thiry, Marc; Cheutin, Thierry; Lamaye, Françoise et al.
2009In Histochemistry and Cell Biology, 132 (2), p. 129-40
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Keywords :
Cell Nucleus/metabolism; Coiled Bodies/metabolism; Fluorescent Antibody Technique; Hela Cells; Humans; Imaging, Three-Dimensional; Interphase; Microscopy, Confocal; Microscopy, Immunoelectron; Mitosis; Nuclear Proteins/analysis/metabolism; Phosphoproteins/analysis/metabolism; RNA Precursors/metabolism; RNA Processing, Post-Transcriptional
Abstract :
[en] We investigated distribution of the nucleolar phosphoprotein Nopp140 within mammalian cells, using immunofluorescence confocal microscopy and immunoelectron microscopy. During interphase, three-dimensional image reconstructions of confocal sections revealed that nucleolar labelling appeared as several tiny spheres organized in necklaces. Moreover, after an immunogold labelling procedure, gold particles were detected not only over the dense fibrillar component but also over the fibrillar centres of nucleoli in untreated and actinomycin D-treated cells. Labelling was also consistently present in Cajal bodies. After pulse-chase experiments with BrUTP, colocalization was more prominent after a 10- to 15-min chase than after a 5-min chase. During mitosis, confocal analysis indicated that Nopp140 organization was lost. The protein dispersed between and around the chromosomes in prophase. From prometaphase to telophase, it was also detected in numerous cytoplasmic nucleolus-derived foci. During telophase, it reappeared in the reforming nucleoli of daughter nuclei. This strongly suggests that Nopp140 could be a component implicated in the early steps of pre-rRNA processing.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Cheutin, Thierry
Lamaye, Françoise ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Thelen, Nicolas  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Meier, U Thomas
O'Donohue, Marie*-Francoise
Ploton, Dominique
Language :
English
Title :
Localization of Nopp140 within mammalian cells during interphase and mitosis.
Publication date :
2009
Journal title :
Histochemistry and Cell Biology
ISSN :
0948-6143
eISSN :
1432-119X
Publisher :
Springer Science & Business Media B.V., New York, United States - New York
Volume :
132
Issue :
2
Pages :
129-40
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 November 2009

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