Article (Scientific journals)
Spontaneous zygogenesis in Escherichia coli, a form of true sexuality in prokaryotes.
Gratia, Jean-Pierre; Thiry, Marc
2003In Microbiology, 149 (Pt 9), p. 2571-84
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Keywords :
Conjugation, Genetic; DNA, Bacterial; Escherichia coli/genetics/metabolism/physiology; F Factor; Microscopy, Electron; Prokaryotic Cells; Recombination, Genetic
Abstract :
[en] A new type of mating, differing from classic conjugation and previously observed in a certain strain of Escherichia coli K-12, has also been found in strains derived from ordinary F- cells of E. coli K-12 exposed to an exogenous factor originating in an E. coli clinical isolate. Immunofluorescence and electron microscopy after single and double labelling of DNA were used to produce evidence in favour of a novel mating mechanism by cell contact at the poles of the bacterial rod. These findings are supported by genetic analyses indicating complete genetic mixing. Unstable complementing diploids were formed, which throw off phenotypically haploid cells, of which some showed a parental phenotype and some were true genetic recombinants. Recombination was observed even when one parent was a UV-inactivated F- RecA- strain. The name 'spontaneous zygogenesis' (Z-mating, for short) is proposed for this kind of mating.
Disciplines :
Microbiology
Author, co-author :
Gratia, Jean-Pierre
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Language :
English
Title :
Spontaneous zygogenesis in Escherichia coli, a form of true sexuality in prokaryotes.
Publication date :
2003
Journal title :
Microbiology
ISSN :
1350-0872
eISSN :
1465-2080
Publisher :
Society for General Microbiology, Reading, United Kingdom
Volume :
149
Issue :
Pt 9
Pages :
2571-84
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 November 2009

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