Reference : Utilization of methyloleate in production of microbial lipase
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biotechnology
Life sciences : Biochemistry, biophysics & molecular biology
Utilization of methyloleate in production of microbial lipase
Destain, Jacqueline mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Fickers, Patrick mailto [Université Libre de Bruxelles - ULB > > Centre d'ingénierie des protéines > > >]
Weekers, F. [> > > >]
Moreau, Benoit [ > > ]
Thonart, Philippe mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Applied Biochemistry and Biotechnology
Humana Press Inc
Yes (verified by ORBi)
[en] Yarrowia lipolytica ; lipase ; bioreactor ; methyloleate ; olive oil ; oleic acid
[en] In this article, we report the development and optimization of an industrial culture medium for the production of extracellular lipase in the yeast Yarrowia lipolytica. Until now olive oil in combination with glucose was used as the carbon source and inducer for the production of lipase. Our results demonstrate that methyloleate, a cheap hydrophobic compound, could efficiently substitute olive oil as the inducer and carbon source for lipase production. A new process of lipase production was developed yielding a twofold increase in the level of production compared with the levels in previous reports.

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