Reference : Involvement of hexokinase HXK1 in glucose catabolite repression of LIP2 encoding extr...
Scientific journals : Article
Life sciences : Microbiology
Involvement of hexokinase HXK1 in glucose catabolite repression of LIP2 encoding extracellular lipase in the yeast Yarrowia lipolytica
Fickers, Patrick mailto [ULB > > > >]
Nicaud, J. M. [> > > >]
Destain, Jacqueline mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Thonart, Philippe mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Current Microbiology
Yes (verified by ORBi)
New York
[en] Yarrowia lipolytica ; lipase ; hexokinase Hxk1
[en] The yeast Yarrowia lipolytica produces an extracellular lipase encoded by the LIP2 gene. However, very little is known about the mechanisms controlling its expression, especially on glucose media. In this work, the involvement of hexokinase Hxk1 in the glucose catabolite repression of LIP2 was investigated in a lipase overproducing mutant less sensitive to glucose repression. This mutant has a reduced capacity to phosphorylate hexose compared with the wild-type strain, but no differences could be observed between the HXK1 sequences in the two isolates. This suggested that the reduced phosphorylating activity of the mutant strain probably resulted from a modification in the level of HXK1 expression. However, overexpression of the HXK1 gene in this mutant led to a decrease of both LIP2 induction and extracellular lipase activity, suggesting that the hexokinase is involved in the glucose catabolite repression of LIP2 in Y lipolytica.

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