Co-diet supplementation of low density polyethylene and honeybee wax did not influence the core gut bacteria and associated enzymes of Galleria mellonella larvae (Lepidoptera: Pyralidae).
Great wax moth; Insect gut microbiome; Insect larvae; Plastic biodegradation; Plastic degrading enzymes; Microbiology; Microbiology (medical)
Abstract :
[en] The current plastic pollution throughout the world is a rising concern that demands the optimization of biodegradation processes. One avenue for this is to identify plastic-degrading bacteria and associated enzymes from the gut bacteria of insect models such as Tenebrio molitor, Plodia interpunctella or Galleria mellonella that have the ability to ingest and rapidly degrade polyethylene. Therefore, this study takes part in understanding the role of the gut bacteria by investigating G. mellonella as a biological model feeding with a diet based on honeybee wax mixed or not with low-density polyethylene. Gut microbiome was analyzed by high throughput 16S rRNA sequencing, and Enterococcaceae and Oxalobacteraceae were found to be the major bacterial families. Compared to the control, the supplementation of low-density polyethylene did not cause significant modification of the bacterial microbiota at community and taxa levels, suggesting bacterial microbiome resilience. The bacterial proteome analysis of gut contents was encouraging for the identification of plastic degrading enzymes such as the phenylacetaldehyde dehydrogenase which participate in styrene degradation. This study allowed a better characterization of the gut bacteria of G. mellonella and provided a basis for the further study of biodegradation of polyethylene based on the bacterial microbiota from insect guts.
Disciplines :
Microbiology
Author, co-author :
Noël, Grégoire ; Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs
Serteyn, Laurent ; Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs
Sare, Abdoul Razack ; Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs
Massart, Sébastien ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs
Delvigne, Frank ; Université de Liège - ULiège > TERRA Research Centre > Microbial technologies
Francis, Frédéric ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs
Language :
English
Title :
Co-diet supplementation of low density polyethylene and honeybee wax did not influence the core gut bacteria and associated enzymes of Galleria mellonella larvae (Lepidoptera: Pyralidae).
Publication date :
2023
Journal title :
International Microbiology
ISSN :
1139-6709
eISSN :
1618-1905
Publisher :
Springer Science and Business Media Deutschland GmbH, Switzerland
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