Reference : Period 2 regulates neural stem/progenitor cell proliferation in the adult hippocampus.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/29788
Period 2 regulates neural stem/progenitor cell proliferation in the adult hippocampus.
English
Borgs, Laurence [Université de Liège - ULg > Département des sciences cliniques > Neurologie >]
Beukelaers, Pierre [Université de Liège - ULg > > GIGA - Neurosciences >]
Vandenbosch, Renaud mailto [Université de Liège - ULg > > GIGA - Neurosciences >]
Nguyen, Laurent [Université de Liège - ULg > Département des sciences cliniques > Neurologie >]
Moonen, Gustave [Centre Hospitalier Universitaire de Liège - CHU > > Neurologie Sart Tilman >]
Maquet, Pierre [Centre Hospitalier Universitaire de Liège - CHU > > Neurologie Sart Tilman >]
Albrecht, Urs [> > > >]
Belachew, Shibeshih mailto [Université de Liège - ULg > Département des sciences cliniques > Neurologie >]
Malgrange, Brigitte [Université de Liège - ULg > > GIGA - Neurosciences - Neurologie >]
2009
BMC Neuroscience
BioMed Central
10
30
Yes
International
1471-2202
[en] Animals ; Blotting, Western ; Cell Cycle Proteins/genetics/metabolism/physiology ; Cell Death/physiology ; Cell Proliferation ; Cells, Cultured ; Dentate Gyrus/cytology/growth & development ; Hippocampus/cytology/growth & development ; Immunohistochemistry ; In Situ Hybridization ; In Situ Nick-End Labeling ; Mice ; Mice, Knockout ; Neurogenesis/genetics/physiology ; Neurons/metabolism ; Nuclear Proteins/deficiency/genetics/metabolism/physiology ; Stem Cells/metabolism ; Transcription Factors/deficiency/genetics/metabolism/physiology
[en] BACKGROUND: Newborn granule neurons are generated from proliferating neural stem/progenitor cells and integrated into mature synaptic networks in the adult dentate gyrus of the hippocampus. Since light/dark variations of the mitotic index and DNA synthesis occur in many tissues, we wanted to unravel the role of the clock-controlled Period2 gene (mPer2) in timing cell cycle kinetics and neurogenesis in the adult DG. RESULTS: In contrast to the suprachiasmatic nucleus, we observed a non-rhythmic constitutive expression of mPER2 in the dentate gyrus. We provide evidence that mPER2 is expressed in proliferating neural stem/progenitor cells (NPCs) and persists in early post-mitotic and mature newborn neurons from the adult DG. In vitro and in vivo analysis of a mouse line mutant in the mPer2 gene (Per2Brdm1), revealed a higher density of dividing NPCs together with an increased number of immature newborn neurons populating the DG. However, we showed that the lack of mPer2 does not change the total amount of mature adult-generated hippocampal neurons, because of a compensatory increase in neuronal cell death. CONCLUSION: Taken together, these data demonstrated a functional link between the constitutive expression of mPER2 and the intrinsic control of neural stem/progenitor cells proliferation, cell death and neurogenesis in the dentate gyrus of adult mice.
Giga-Neurosciences
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Léon Fredericq
Researchers
http://hdl.handle.net/2268/29788
also: http://hdl.handle.net/2268/81306 ; http://hdl.handle.net/2268/95470
10.1186/1471-2202-10-30

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
BMC neuroscience (2009) - borgs.pdfPublisher postprint1.61 MBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.