deoxynivalenol; biological detoxification; DON derivatives; Bacillus sp
Abstract :
[en] Deoxynivalenol (DON), produced by Fusarium species, is one of the most common trichothecenes detected in cereals pre- and post-harvest, which poses a great threat to the health of livestock and human beings due to its strong toxicity. In this study, we isolated and characterized two DON-degrading bacterial strains, Bacillus sp. HN117 and Bacillus sp. N22. Both strains could degrade DON efficiently in a wide range of temperatures (from 25 °C to 42 °C) and concentrations (from 10 mg/L to 500 mg/L). After optimization of the degradation conditions, 29.0% DON was eliminated by HN117 in 72 h when it was incubated with 1000 mg/L DON; meanwhile, the DON degradation rate of N22 was boosted notably from 7.41% to 21.21% within 120 h at 500 mg/L DON. Degradation products analysis indicated HN117 was able to transform DON into a new isomer M-DOM, the possible structure of which was deduced based on LC-MS and NMR analysis, and N22 could convert DON into potential low-toxic derivatives norDON E and 9-hydroxymethyl DON lactone. These two strains have the potential to be developed as new biodegrading agents to control DON contamination in food and feed industries.
Disciplines :
Entomology & pest control
Author, co-author :
Li, Beibei ; Université de Liège - ULiège > TERRA Research Centre
Duan, Jiaqi
Ren, Jie
Francis, Frédéric ; Université de Liège - ULiège > TERRA Research Centre > Gestion durable des bio-agresseurs
Li, Guangyue
Language :
English
Title :
Isolation and Characterization of Two New Deoxynivalenol-Degrading Strains, Bacillus sp. HN117 and Bacillus sp. N22
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