Ultrastructural methods for nucleic acid detection by immunocytology.

Thiry, Marc

doi:10.1016/s0079-6336(99)80008-4

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Ultrastructural methods for nucleic acid detection by immunocytology.

Thiry, Marc

1999 • In Progress in Histochemistry and Cytochemistry, 34 (2), p. 87-159

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https://hdl.handle.net/2268/29332

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10.1016/s0079-6336(99)80008-4

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10546283

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Keywords :

Animals; Antibodies, Monoclonal; Bromodeoxyuridine; DNA/analysis/immunology/ultrastructure; DNA Nucleotidylexotransferase; Humans; Immunohistochemistry/methods; In Situ Nick-End Labeling; Microscopy, Immunoelectron/methods; Nucleic Acids/analysis/immunology/ultrastructure; RNA/analysis/immunology/ultrastructure

Abstract :

[en] In the present review are summarized recent developments in immunocytochemical detection of nucleic acids in biological materials at the ultrastructural level. Not only the approaches using antibodies to natural nucleic acids are described but also the techniques involving the use of antibodies raised against various nucleotide analogs incorporated beforehand into nucleic acids. Special emphasis is placed on each method's potential and limitations. These methods, combined or not with molecular biotechnology, are powerful tools for studying the structure and function of nucleic acids. They can be used to investigate the distribution and topological organization of DNA and RNA molecules or of specialized within these molecules in the cells.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Thiry, Marc ; Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire

Language :

English

Title :

Ultrastructural methods for nucleic acid detection by immunocytology.

Publication date :

1999

Journal title :

Progress in Histochemistry and Cytochemistry

ISSN :

0079-6336

Publisher :

Urban & Fischer, Jena, Germany

Volume :

Issue :

Pages :

87-159

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Peer Reviewed verified by ORBi

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since 25 November 2009

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