Reference : Ultrastructural methods for nucleic acid detection by immunocytology.
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/29332
Ultrastructural methods for nucleic acid detection by immunocytology.
English
Thiry, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
1999
Progress in Histochemistry and Cytochemistry
Urban & Fischer
34
2
87-159
Yes (verified by ORBi)
International
0079-6336
Jena
Germany
[en] Animals ; Antibodies, Monoclonal ; Bromodeoxyuridine ; DNA/analysis/immunology/ultrastructure ; DNA Nucleotidylexotransferase ; Humans ; Immunohistochemistry/methods ; In Situ Nick-End Labeling ; Microscopy, Immunoelectron/methods ; Nucleic Acids/analysis/immunology/ultrastructure ; RNA/analysis/immunology/ultrastructure
[en] In the present review are summarized recent developments in immunocytochemical detection of nucleic acids in biological materials at the ultrastructural level. Not only the approaches using antibodies to natural nucleic acids are described but also the techniques involving the use of antibodies raised against various nucleotide analogs incorporated beforehand into nucleic acids. Special emphasis is placed on each method's potential and limitations. These methods, combined or not with molecular biotechnology, are powerful tools for studying the structure and function of nucleic acids. They can be used to investigate the distribution and topological organization of DNA and RNA molecules or of specialized within these molecules in the cells.
http://hdl.handle.net/2268/29332
also: http://hdl.handle.net/2268/29349

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