No full text
Article (Scientific journals)
Ultrastructural distribution of DNA within plant meristematic cell nucleoli during activation and the subsequent inactivation by a cold stress.
Mineur, Pierre; Jennane, A.; Thiry, Marc et al.
1998In Journal of Structural Biology, 123 (3), p. 199-210
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Keywords :
Cell Nucleolus/ultrastructure; Chromatin/ultrastructure; Cold Temperature; DNA/analysis/ultrastructure; DNA Nucleotidylexotransferase/metabolism; Genes, Plant/genetics; Germination; Immunohistochemistry; Microscopy, Immunoelectron; Peas/ultrastructure; RNA, Ribosomal/genetics; Zea mays/ultrastructure
Abstract :
[en] We have investigated the precise location of DNA within the meristematic cell nucleolus of Zea mays root cells and Pisum sativum cotyledonary buds, in the course of their activation and induced inactivation following a subsequent treatment at low temperature. For this purpose, we combined the acetylation method, providing an excellent distinction between the various nucleolar components, with the in situ terminal deoxynucleotidyl transferase-immunogold technique, a highly sensitive method for detecting DNA at the ultrastructural level. In addition to the presence of DNA in the condensed chromatin associated with the nucleolus, we demonstrated that a significant label was detected in the nucleolus of quiescent cells in both plant models. Evident labels were also found in the dense fibrillar component of actived nucleoli. Whereas in inactivated nucleoli no significant label was observed within the dense fibrillar component, an intense label was seen over the large heterogeneous fibrillar centres only during inactivation. The granular component was never significantly labelled. These results appear to indicate that the DNA present in the dense fibrillar component of activated nucleoli withdraws from this structure during its inactivation and becomes incorporated in the large fibrillar centres. These observations suggest that in plant cells inactivation of rRNA genes is clearly accompanied by changes in the conformation of ribosomal chromatin.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Mineur, Pierre ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
Jennane, A.
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Deltour, Roger ;  Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Goessens, Guy ;  Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Language :
English
Title :
Ultrastructural distribution of DNA within plant meristematic cell nucleoli during activation and the subsequent inactivation by a cold stress.
Publication date :
1998
Journal title :
Journal of Structural Biology
ISSN :
1047-8477
eISSN :
1095-8657
Publisher :
Academic Press, San Diego, United States - California
Volume :
123
Issue :
3
Pages :
199-210
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright 1998 Academic Press.
Available on ORBi :
since 25 November 2009

Statistics


Number of views
129 (2 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
22
Scopus citations®
without self-citations
19
OpenCitations
 
15

Bibliography


Similar publications



Contact ORBi