Reference : Ultrastructural distribution of DNA within plant meristematic cell nucleoli during activ...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/29328
Ultrastructural distribution of DNA within plant meristematic cell nucleoli during activation and the subsequent inactivation by a cold stress.
English
Mineur, Pierre mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs >]
Jennane, A. [> > > >]
Thiry, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
Deltour, Roger mailto [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]
Goessens, Guy mailto [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]
1998
Journal of Structural Biology
Academic Press
123
3
199-210
Yes (verified by ORBi)
International
1047-8477
1095-8657
San Diego
CA
[en] Cell Nucleolus/ultrastructure ; Chromatin/ultrastructure ; Cold Temperature ; DNA/analysis/ultrastructure ; DNA Nucleotidylexotransferase/metabolism ; Genes, Plant/genetics ; Germination ; Immunohistochemistry ; Microscopy, Immunoelectron ; Peas/ultrastructure ; RNA, Ribosomal/genetics ; Zea mays/ultrastructure
[en] We have investigated the precise location of DNA within the meristematic cell nucleolus of Zea mays root cells and Pisum sativum cotyledonary buds, in the course of their activation and induced inactivation following a subsequent treatment at low temperature. For this purpose, we combined the acetylation method, providing an excellent distinction between the various nucleolar components, with the in situ terminal deoxynucleotidyl transferase-immunogold technique, a highly sensitive method for detecting DNA at the ultrastructural level. In addition to the presence of DNA in the condensed chromatin associated with the nucleolus, we demonstrated that a significant label was detected in the nucleolus of quiescent cells in both plant models. Evident labels were also found in the dense fibrillar component of actived nucleoli. Whereas in inactivated nucleoli no significant label was observed within the dense fibrillar component, an intense label was seen over the large heterogeneous fibrillar centres only during inactivation. The granular component was never significantly labelled. These results appear to indicate that the DNA present in the dense fibrillar component of activated nucleoli withdraws from this structure during its inactivation and becomes incorporated in the large fibrillar centres. These observations suggest that in plant cells inactivation of rRNA genes is clearly accompanied by changes in the conformation of ribosomal chromatin.
http://hdl.handle.net/2268/29328
10.1006/jsbi.1998.4038
Copyright 1998 Academic Press.

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