Reference : "Exolysosomes", Enzyme-Containing Vesicles in the Ecdysial Space of Molting Crabs
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/29325
"Exolysosomes", Enzyme-Containing Vesicles in the Ecdysial Space of Molting Crabs
English
Compère, Philippe mailto [Université de Liège - ULg > Département des sciences et gestion de l'environnement > Département des sciences et gestion de l'environnement >]
Bouchtia, Hicham [> > > >]
Thiry, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
Goffinet, Gerhard mailto [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]
1997
Journal of Structural Biology
Academic Press
119
3
247-59
Yes (verified by ORBi)
International
1047-8477
1095-8657
San Diego
CA
[en] Exolysosomes ; crab ; moult
[en] Free vesicle-like bodies (VLBs) present in the ecdysial space of cuticle regions undergoing degradation during preecdysis of the Atlantic shore crab Carcinus maenas have been interpreted either as infectious organisms or as secretion structures associated with degradation of the old cuticle. Ultrastructural, cytochemical, and immunocytological investigations were performed to test these hypotheses and to see whether VLBs are peculiar to this crab species. Similar VLBs were systematically found in two other preecdysial crabs, Cancer pagurus and Macropipus puber. In Car, maenas, they originate during early premolt inside Golgi buddings and are often gathered into large vacuoles in epidermal cells. The histochemical azo-dye technique and a cerium-based cytochemical method revealed acid phosphatase activity in both the ecdysial space and the VLBs, while Feulgen's method and immunocytological labeling always failed to reveal any DNA or RNA in either the ecdysial space or the VLBs. We conclude that VLBs are not infectious organisms but "extracellular" cuticle-degrading organelles of lysosomal origin and propose to coin them "exolysosomes."
http://hdl.handle.net/2268/29325
also: http://hdl.handle.net/2268/77118
10.1006/jsbi.1997.3876

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