Reference : Effect of high osmolarity acclimation on tolerance to hyperosmotic shocks in L929 cul...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/29307
Effect of high osmolarity acclimation on tolerance to hyperosmotic shocks in L929 cultured cells.
English
Gilles, Raymond mailto [Université de Liège - ULg > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences) >]
Belkhir, M. [> > > >]
Compère, Philippe mailto [Université de Liège - ULg > Département des sciences et gestion de l'environnement > Département des sciences et gestion de l'environnement >]
Libioulle, Cécile mailto [Centre Hospitalier Universitaire de Liège - CHU > > Génétique >]
Thiry, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie cellulaire >]
1995
Tissue & Cell
Churchill Livingstone, Inc.
27
6
679-687
Yes (verified by ORBi)
International
0040-8166
Edinburgh
Scotland
[en] Animals ; Cell Line ; Cell Survival ; Chromatin/ultrastructure ; Male ; Mice ; Mice, Inbred C3H ; Microscopy, Electron ; Osmolar Concentration ; Osmotic Pressure ; Proline/metabolism ; Sodium Chloride/pharmacology ; Sorbitol/metabolism
[en] Application of abrupt, large hyperosmotic shocks induces in L929 cultured cells changes similar to those previously described in other cell types, notably a hypercondensation of the nuclear chromatin. This paper shows that; 1) this phenomenon is concomitant with a complete disappearance of deoxyribonucleic acid, as visualized by immunogold labelling, from the nucleoplasmic spaces; 2) acclimation to high osmolarities (600 mOsm) by addition to the culture medium of NaCl, sorbitol or proline protects the cells from these effects, which appear to be largely attenuated-acclimated cells also survive much better to the osmotic shock than do control cells and; 3) the best protection seems to be provided by sorbitol and NaCl. Proline acclimation is less effective. These effects are discussed in terms of increased tolerance to NaCl load induced at the level of different macromolecules by so-called 'compensatory' organic compounds.
http://hdl.handle.net/2268/29307

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