No full text
Article (Scientific journals)
Ultrastructural distribution of DNA within the ring-shaped nucleolus of human resting T lymphocytes.
Vandelaer, M.; Thiry, Marc; Goessens, Guy
1993In Experimental Cell Research, 205 (2), p. 430-2
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Keywords :
Cell Nucleolus/ultrastructure; Chromatin/ultrastructure; DNA/metabolism; Humans; T-Lymphocytes/ultrastructure
Abstract :
[en] The precise distribution of DNA within the resting human T lymphocyte nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. The nucleolus is partially enveloped by a layer of condensed chromatin which, at several places, penetrates into the nucleolar body until in close contact with the fibrillar centers. Morphometric analysis reveals that 32% of the fibrillar center surface is essentially occupied by condensed chromatin. Using the in situ terminal deoxynucleotidyl transferase-immunogold procedure for detecting DNA, we further show that evident label is exclusively found over the condensed chromatin and over the fibrillar centers, whereas no significant label is detected over the dense fibrillar component of the nucleolus.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Vandelaer, M.
Thiry, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire
Goessens, Guy ;  Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Language :
English
Title :
Ultrastructural distribution of DNA within the ring-shaped nucleolus of human resting T lymphocytes.
Publication date :
1993
Journal title :
Experimental Cell Research
ISSN :
0014-4827
eISSN :
1090-2422
Publisher :
Academic Press, Orlando, United States - Florida
Volume :
205
Issue :
2
Pages :
430-2
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 November 2009

Statistics


Number of views
42 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
21
Scopus citations®
without self-citations
17
OpenCitations
 
11

Bibliography


Similar publications



Contact ORBi